共 21 条
MOLECULAR ANALYSIS OF A BJERKANDERA-ADUSTA LIGNIN PEROXIDASE GENE
被引:4
作者:
KIMURA, Y
[1
]
ASADA, Y
[1
]
OKA, T
[1
]
KUWAHARA, M
[1
]
机构:
[1] KAGAWA UNIV,FAC AGR,DEPT BIORESOURCE SCI,MIKI,KAGAWA 76107,JAPAN
关键词:
D O I:
暂无
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
A cDNA clone, lambda-LPO-1, encoding a major lignin peroxidase from the basidiomycete Bjerkandera adusta was isolated and characterized. The nucleotide sequence of lambda-LPO-1 predicts a mature protein consisting of 349 amino acids with a molecular weight of 37,225 preceded by a signal peptide of 23 amino acid residues. We have also cloned and sequenced the gene encoding lignin peroxidase from B. adusta. Comparison of these sequences reveals a lignin peroxidase gene structure consisting of 1,116 bp of protein-encoding DNA that is interrupted by four intervening sequences. The putative eukaryotic regulatory sequence, a TATA box, is present at position -75 relative to the translational initiation codon. Amino acid sequence homology between the coding regions of lambda-LPO-1 and of the lignin peroxidase cDNA clone lambda-ML-1 from Phanerochaete chrysosporium is 61%.
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页码:510 / 514
页数:5
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