MOLECULAR ANALYSIS OF A BJERKANDERA-ADUSTA LIGNIN PEROXIDASE GENE

被引：4

作者：

KIMURA, Y ^{[1
]}

ASADA, Y ^{[1
]}

OKA, T ^{[1
]}

KUWAHARA, M ^{[1
]}

机构：

[1] KAGAWA UNIV,FAC AGR,DEPT BIORESOURCE SCI,MIKI,KAGAWA 76107,JAPAN

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1991年 / 35卷 / 04期

关键词：

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cDNA clone, lambda-LPO-1, encoding a major lignin peroxidase from the basidiomycete Bjerkandera adusta was isolated and characterized. The nucleotide sequence of lambda-LPO-1 predicts a mature protein consisting of 349 amino acids with a molecular weight of 37,225 preceded by a signal peptide of 23 amino acid residues. We have also cloned and sequenced the gene encoding lignin peroxidase from B. adusta. Comparison of these sequences reveals a lignin peroxidase gene structure consisting of 1,116 bp of protein-encoding DNA that is interrupted by four intervening sequences. The putative eukaryotic regulatory sequence, a TATA box, is present at position -75 relative to the translational initiation codon. Amino acid sequence homology between the coding regions of lambda-LPO-1 and of the lignin peroxidase cDNA clone lambda-ML-1 from Phanerochaete chrysosporium is 61%.

引用

页码：510 / 514

页数：5

共 21 条

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