HYPERPHOSPHORYLATION OF P53 INDUCED BY BENZENE, TOLUENE, AND CHLOROFORM

被引：17

作者：

DEES, C

TRAVIS, C

机构：

[1] Molecular Toxicology Group, Risk Analysis Section, Health Sciences Research Division, Oak Ridge, TN 37831-6109, Bldg. 4500S/MS-6109

来源：

CANCER LETTERS | 1994年 / 84卷 / 02期

关键词：

P53; TUMOR PROMOTER; PROTEIN KINASE C; PHOSPHORYLATION;

D O I：

10.1016/0304-3835(94)90365-4

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Phorbol 12-myristate, 13-acetate (PMA) is a known protein kinase C activator (PKC); benzene, chloroform, and toluene have also been reported to be PKC activators. We examined the effects of these three solvents on the phosphorylation of p53 in treated cells. Hyperphosphorylated p53 was found when p53 was immunoprecipitated from rat liver epithelial cell extracts treated with any of the solvents or PMA. The solvents also resulted in hyper-phosphorylation of human p53 produced by transfection of Saos-2 cells with a eucaryotic expression vector. Increased phosphorylation of p53 induced by the solvents was also observed through in vitro assays. Hyperphosphoryation of p53 may be involved in tumor promotion by benzene, toluene and chloroform.

引用

页码：117 / 123

页数：7

共 17 条

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