DETERMINATION OF BETA-CYCLODEXTRIN INCLUSION COMPLEX CONSTANTS FOR 3,4-DIHYDRO-2-H-1-BENZOPYRAN ENANTIOMERS BY CAPILLARY ELECTROPHORESIS

被引：49

作者：

BAUMY, P ^{[1
]}

MORIN, P ^{[1
]}

DREUX, M ^{[1
]}

VIAUD, MC ^{[1
]}

BOYE, S ^{[1
]}

GUILLAUMET, G ^{[1
]}

机构：

[1] UNIV ORLEANS,CHIM BIOORGAN & ANALYT LAB,CNRS,URA 499,F-45067 ORLEANS 2,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 707卷 / 02期

关键词：

D O I：

10.1016/0021-9673(95)00325-H

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chiral separation of 3,4-dihydro-2H-1-benzopyran derivatives by capillary zone electrophoresis was achieved by employing beta-cyclodextrin (beta-CD) as chiral selector. The effects of electrolyte composition (beta-cyclodextrin concentration, ionic strength and pH of the buffer) on the migration time, enantioselectivity, peak efficiency and resolution were investigated. As expected, there was an optimum beta-CD concentration (C-opt) which gave maximum enantioselectivity. The stability constant for the beta-CD inclusion complex was determined for each enantiomer of two 3,4-dihydro-2H-1-benzopyran derivatives. For each solute, the experimental value of C-opt agreed well with the value calculated from the equation [C](opt) = 1/(KRKS)(1/2), where we used experimental values for the inclusion complex constants (K-R,K-S). The enantiomeric separation of three 3,4-dihydro-2H-1-benzopyran derivatives was achieved using this optimization method, and baseline separation was obtained in less than 15 min with an efficiency of between 300 000 and 600 000 theoretical plates.

引用

页码：311 / 326

页数：16