PURIFICATION, CHARACTERIZATION, CRYSTALLIZATION, AND PRELIMINARY-X-RAY RESULTS FROM PARACOCCUS-DENITRIFICANS PORIN

被引：10

作者：

HIRSCH, A

WACKER, T

WECKESSER, J

DIEDERICHS, K

WELTE, W

机构：

[1] UNIV KONSTANZ,FAK BIOL,D-78434 CONSTANCE,GERMANY

[2] INST BIOPHYS & STRAHLENBIOL,D-79104 FREIBURG,GERMANY

[3] INST BIOL 2,D-79104 FREIBURG,GERMANY

来源：

PROTEINS-STRUCTURE FUNCTION AND GENETICS | 1995年 / 23卷 / 02期

关键词：

CRYSTALS; MEMBRANE PROTEIN; OUTER MEMBRANE; X-RAY DIFFRACTION;

D O I：

10.1002/prot.340230217

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The porin from Paracoccus denitrificans ATCC 13543 was purified and crystallized. Two crystal forms were obtained from porin solutions with beta-d-octylglucopyranoside as detergent. Crystals of form I belong to the monoclinic spacegroup C2 with unit cell dimensions a = 112.2 Angstrom, b = 193.8 Angstrom, c = 100.5 Angstrom and beta = 129.2 degrees. There is 1 trimer per asymmetric unit. Crystals of form II are triclinic with a = 89.7 Angstrom, b = 98.8 Angstrom, c = 112.5 Angstrom, alpha = 112.5 degrees, beta = 101.8 degrees, gamma = 106.7 degrees (2 trimers per asymmetric unit). Both crystal forms diffract to 3 Angstrom. (C) 1995 Wiley-Liss, Inc.

引用

页码：282 / 284

页数：3

共 19 条

[1] SELECTIVITY CHANGES IN SITE-DIRECTED MUTANTS OF THE VDAC ION CHANNEL - STRUCTURAL IMPLICATIONS [J].