ASSAY OF PROTEIN-A IN STAPHYLOCOCCUS-HYICUS SUBSP HYICUS BY ELISA AND IMMUNOELECTRON MICROSCOPY

被引：6

作者：

TAKEUCHI, S ^{[1
]}

KOBAYASHI, Y ^{[1
]}

MORI, Y ^{[1
]}

机构：

[1] NATL INST ANIM HLTH,TSUKUBA,IBARAKI 305,JAPAN

来源：

VETERINARY MICROBIOLOGY | 1990年 / 25卷 / 2-3期

关键词：

D O I：

10.1016/0378-1135(90)90085-A

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The presence and quantity of protein A in Staphylococcus hyicus subsp. hyicus isolates were examined by an enzyme-linked immunosorbent assay (ELISA) and immunoelectron microscopy. Cell-bound protein A was demonstrated in 45 (94%) of 48 isolates from diseased pigs and in 113 (86%) of 132 isolates from healthy pigs by ELISA using peroxidase-conjugated rabbit antibody, but was not found in isolates from chickens and cows. Most of these swine isolates contained about 100 to 300 ng of cell-bound protein A/ml. Extracellular protein A was not detected in any isolates from pigs, chickens or cows. In the immunoelectron microscopy assay, swine isolates were labeled with goat anti-mouse IgG conjugated to colloidal gold particles, but chicken and cow isoaltes were not labeled. © 1990.

引用

页码：297 / 302

页数：6

共 17 条

[1]

COX HU, 1986, AM J VET RES, V47, P1881

[2] STAPHYLOCOCCUS-HYICUS IN CATTLE [J].