A GENERAL VECTOR, PASK84, FOR CLONING, BACTERIAL PRODUCTION, AND SINGLE-STEP PURIFICATION OF ANTIBODY F-AB FRAGMENTS

被引：83

作者：

SKERRA, A

机构：

[1] Max-Planck-lnstitut für Biophysik, Frankfurt am Main

来源：

GENE | 1994年 / 141卷 / 01期

关键词：

ESCHERICHIA COLI; IMMUNOGLOBULIN; SECRETION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMINODIACETIC ACID SEPHAROSE; OLIGO-HISTIDINE TAG;

D O I：

10.1016/0378-1119(94)90131-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The expression vector pASK84 was designed for the convenient cloning of immunoglobulin variable domain genes, as well as periplasmic secretion of the corresponding F-ab fragment in Escherichia coli. The plasmid provides the constant domain genes of mouse IgG1/K with a hexa-histidine tag fused to the C terminus of the heavy chain. This strategy enables the rapid and efficient purification of the functional recombinant F-ab fragment via immobilized metal affinity chromatography. The versatility of this expression and purification system is demonstrated using the variable domains of the well-characterized anti-lysozyme antibody D1.3.

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页码：79 / 84

页数：6

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