CRYSTALS OF SOLUBLE INTERLEUKIN-1 RECEPTOR COMPLEXED WITH ITS NATURAL ANTAGONIST REVEAL A 1/1-RECEPTOR-LIGAND COMPLEX

被引：5

作者：

SCHREUDER, H

TARDIF, C

SOFFIENTINI, A

SARUBBI, E

AKESON, A

BOWLIN, T

YANOFSKY, S

BARRETT, RW

机构：

[1] LEPETIT RES CTR,I-21040 GERENZANO,ITALY

[2] MARION MERRELL DOW RES INST,CINCINNATI,OH 45242

[3] AFFYMAX RES INST,PALO ALTO,CA 94304

来源：

FEBS LETTERS | 1995年 / 373卷 / 01期

关键词：

INTERLEUKIN-1; RECEPTOR; INTERLEUKIN-1 RECEPTOR ANTAGONIST; CYTOKINE; PROTEIN CRYSTAL; X-RAY ANALYSIS; HUMAN;

D O I：

10.1016/0014-5793(95)01009-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interleukin-1 is a cytokine involved in the acute phase response against infection and injury, We obtained crystals of a complex of soluble, recombinant human interleukin-l receptor and recombinant human interleukin-l receptor antagonist, a naturally occurring antagonist. The crystals are suitable for X-ray analysis and diffract to 2.7 Angstrom resolution, Solvent content calculations indicate that the crystals contain one receptor and one antagonist molecule per asymmetric unit, Other receptor to antagonist ratios are highly unlikely, These results suggest that the interleukin-1 antagonist binds a single receptor molecule and does not cause receptor aggregation.

引用

页码：39 / 40

页数：2

共 19 条

[1]

BANNER DW, CELL, V73, P431

[2] SEQUENCE OF THE CDNA FOR THE HUMAN FIBROBLAST TYPE INTERLEUKIN-1 RECEPTOR [J].