A NOVEL HPLC METHOD FOR GLUTATHIONE-INSULIN TRANSHYDROGENASE ASSAY USING FLUORESCENCE LABELED INSULIN

被引：2

作者：

SAITO, N

YAMANAKA, H

NAGASAWA, S

机构：

[1] National Institute of Animal Health, Tsukuba, Ibaraki 305

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1991年 / 53卷 / 01期

关键词：

FLUORESCEIN-THIOCARBAMYLATED INSULIN; GLUTATHIONE; GLUTATHIONE-INSULIN TRANSHYDROGENASE; HPLC; INSULIN;

D O I：

10.1292/jvms.53.1

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The activity of rat liver glutathione-insulin transhydrogenase (GIT) was measured by HPLC. The degradation of fluorescein isothiocyanate-I (FITC-I)-labeled insulin is separated into several peaks, which are bound different amount of FITC-I. We selected mono-fluorescein-thiocarbamylated insulin to estimate the decrease of insulin content and it became possible to assay GIT activity. This novel method was time-saving and simple, and this system could utilize instead of previous method. © 1991, JAPANESE SOCIETY OF VETERINARY SCIENCE. All rights reserved.

引用

页码：1 / 4

页数：4

共 21 条

[1] POSTPRANDIAL PLASMA GLUCOSE, INSULIN, GLUCAGON AND TRIGLYCERIDE RESPONSES TO A STANDARD DIET IN NORMAL SUBJECTS [J].