THE AMINO-ACID-SEQUENCE OF NUCLEOSIDE DIPHOSPHATE KINASE-I FROM SPINACH LEAVES, AS DEDUCED FROM THE CDNA SEQUENCE

被引：47

作者：

NOMURA, T ^{[1
]}

YATSUNAMI, K ^{[1
]}

HONDA, A ^{[1
]}

SUGIMOTO, Y ^{[1
]}

FUKUI, T ^{[1
]}

ZHANG, J ^{[1
]}

YAMAMOTO, J ^{[1
]}

ICHIKAWA, A ^{[1
]}

机构：

[1] KYOTO UNIV,FAC PHARMACEUT SCI,DEPT PHYSIOL CHEM,SAKYO KU,KYOTO 606,JAPAN

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1992年 / 297卷 / 01期

关键词：

D O I：

10.1016/0003-9861(92)90638-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The primary structure of nucleoside diphosphate (NDP) kinase from spinach leaves has been deduced from its cDNA sequence. A λgt11 cDNA library derived from spinach leaves was screened using an antibody against NDP kinase I, which we previously purified to electrophoretic homogeneity (T. Nomura, T. Fukui, and A. Ichikawa, 1991, Biochim. Biophys. Acta 1077, 47-55). The cDNA sequences of positive clones contained the amino acid coding region (444 base pairs) for NDP kinase I as well as 5′ and 3′ noncoding regions of 33 and 361 base pairs, respectively. The cDNAs hybridized to a 1.1-kb mRNA. NDP kinase I contains 148 amino acid residues with a molecular mass of 16,305, which is in excellent agreement with that of the purified enzyme (16 kDa). Homology was found between the sequence of spinach NDP kinase I and those of the rat, Myxococcus xanthus, and Dictyostelium discoideum NDP kinases, as well as the human Nm23-gene product and the awd protein of Drosophila melanogaster. © 1992.

引用

页码：42 / 45

页数：4

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