A NEW FLOW CYTOMETRIC ASSAY FOR THE EVALUATION OF PHAGOCYTOSIS AND THE OXIDATIVE BURST IN WHOLE-BLOOD

被引：76

作者：

PERTICARARI, S ^{[1
]}

PRESANI, G ^{[1
]}

BANFI, E ^{[1
]}

机构：

[1] UNIV TRIESTE,IST MICROBIOL,I-34127 TRIESTE,ITALY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 170卷 / 01期

关键词：

FLOW CYTOMETRY; PHAGOCYTOSIS; REACTIVE OXYGEN INTERMEDIATE;

D O I：

10.1016/0022-1759(94)90251-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phagocytes play an important role in host defence against microorganisms and different techniques are needed to evaluate their functional activities. Here we describe a rapid, simple and reliable one step procedure to measure the phagocytosis rate and oxidative burst activation of both polymorphonuclear leukocytes (PMN) and monocytes, by means of flow cytometry using only small quantities of whole blood. The two species of bacteria employed as test microorganisms, Staphylococcus aureus and Candida albicans showed a somewhat different behaviour. We found that the present method could be used as an alternative test in the diagnosis of chronic granulomatous disease (CGD). Moreover we were able to analyse, in a one step procedure, defective phagocyte functions in a group of paediatric patients suffering from recurrent microbial infections.

引用

页码：117 / 124

页数：8

共 19 条

[1] RAPID FLOW CYTOMETRIC STUDIES OF BORRELIA-BURGDORFERI PHAGOCYTOSIS BY HUMAN POLYMORPHONUCLEAR LEUKOCYTES
BANFI, E
CINCO, M
PERTICARARI, S
PRESANI, G
[J]. JOURNAL OF APPLIED BACTERIOLOGY, 1989, 67 (01): : 37 - 45
[2] BASS DA, 1983, J IMMUNOL, V130, P1910
[3] BJERKNES R, 1983, ACTA PATH MICRO IM C, V91, P341
[4] DOSE EFFECTS OF LPS ON NEUTROPHILS IN A WHOLE-BLOOD FLOW CYTOMETRIC ASSAY OF PHAGOCYTOSIS AND OXIDATIVE BURST
BOHMER, RH
TRINKLE, LS
STANECK, JL
[J]. CYTOMETRY, 1992, 13 (05): : 525 - 531
[5] STAPHYLOCOCCUS-AUREUS PHAGOCYTOSIS - A NEW CYTOFLUOROMETRIC METHOD USING FITC AND PARAFORMALDEHYDE
CANTINIEAUX, B
HARIGA, C
COURTOY, P
HUPIN, J
FONDU, P
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 121 (02) : 203 - 208
[6] SIMULTANEOUS EVALUATION OF PHAGOCYTOSIS AND FC-GAMMA-R-MEDIATED OXIDATIVE BURST IN HUMAN MONOCYTES BY A SIMPLE FLOW-CYTOMETRY METHOD
CASADO, JA
MERINO, J
CID, J
SUBIRA, ML
SANCHEZIBARROLA, A
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1993, 159 (1-2) : 173 - 176
[7] DUNN PA, 1981, J LAB CLIN MED, V98, P374
[8] A FAST AND EASY METHOD TO DETERMINE THE PRODUCTION OF REACTIVE OXYGEN INTERMEDIATES BY HUMAN AND MURINE PHAGOCYTES USING DIHYDRORHODAMINE-123
EMMENDORFFER, A
HECHT, M
LOHMANNMATTHES, ML
ROESLER, J
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 131 (02) : 269 - 275
[9] NEW, SIMPLE FLOW-CYTOMETRY TECHNIQUE TO DISCRIMINATE BETWEEN INTERNALIZED AND MEMBRANE-BOUND PARTICLES IN PHAGOCYTOSIS
FATTOROSSI, A
NISINI, R
PIZZOLO, JG
DAMELIO, R
[J]. CYTOMETRY, 1989, 10 (03): : 320 - 325
[10] SIMULTANEOUS MEASUREMENT BY FLOW-CYTOMETRY OF PHAGOCYTOSIS AND HYDROGEN-PEROXIDE PRODUCTION OF NEUTROPHILS IN WHOLE-BLOOD
HASUI, M
HIRABAYASHI, Y
KOBAYASHI, Y
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 117 (01) : 53 - 58

← 1 2 →