A NEW FLOW CYTOMETRIC ASSAY FOR THE EVALUATION OF PHAGOCYTOSIS AND THE OXIDATIVE BURST IN WHOLE-BLOOD

被引：76

作者：

PERTICARARI, S ^{[1
]}

PRESANI, G ^{[1
]}

BANFI, E ^{[1
]}

机构：

[1] UNIV TRIESTE,IST MICROBIOL,I-34127 TRIESTE,ITALY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 170卷 / 01期

关键词：

FLOW CYTOMETRY; PHAGOCYTOSIS; REACTIVE OXYGEN INTERMEDIATE;

D O I：

10.1016/0022-1759(94)90251-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phagocytes play an important role in host defence against microorganisms and different techniques are needed to evaluate their functional activities. Here we describe a rapid, simple and reliable one step procedure to measure the phagocytosis rate and oxidative burst activation of both polymorphonuclear leukocytes (PMN) and monocytes, by means of flow cytometry using only small quantities of whole blood. The two species of bacteria employed as test microorganisms, Staphylococcus aureus and Candida albicans showed a somewhat different behaviour. We found that the present method could be used as an alternative test in the diagnosis of chronic granulomatous disease (CGD). Moreover we were able to analyse, in a one step procedure, defective phagocyte functions in a group of paediatric patients suffering from recurrent microbial infections.

引用

页码：117 / 124

页数：8

共 19 条

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