INTERACTION OF AMINOGLYCOSIDES WITH THE OUTER MEMBRANES AND PURIFIED LIPOPOLYSACCHARIDE AND OMPF PORIN OF ESCHERICHIA-COLI

被引:127
作者
HANCOCK, REW
FARMER, SW
LI, ZS
POOLE, K
机构
[1] Department of Microbiology, University of British Columbia, Vancouver, BC
关键词
D O I
10.1128/AAC.35.7.1309
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The mechanism of uptake of aminoglycosides across the outer membrane of Escherichia coli was reevaluated. Porin-deficient mutants showed no alteration in gentamicin or kanamycin susceptibility. Furthermore, the influence of kanamycin on intrinsic tryptophan fluorescence of porin OmpF (Y. Kobayashi, and T. Nakae, Eur. J. Biochem. 151:231-236, 1985) was shown to be strongly influenced by protein concentration and EDTA. This led to the hypothesis that aminoglycoside-mediated increases and decreases in intrinsic tryptophan fluorescence were due to aggregation-disaggregation of OmpF mediated by interaction at a divalent cation binding site on OmpF. Gentamicin, kanamycin, and polymyxin B increased E. coli outer membrane permeability to the hydrophobic fluorescent compound 1-N-phenyl-naphthylamine (NPN) and the peptidoglycan-degrading enzyme lysozyme. Addition of Mg2+ blocked these permeabilizing activities. Furthermore, gentamicin and polymyxin B bound to Mg2+-binding sites on E. coli lipopolysaccharide, as determined in dansyl polymyxin displacement experiments. A polymyxin-resistant, lipopolysaccharide-altered pmr mutant of E. coli had a fourfold-lower MIC of gentamicin and kanamycin and was more poorly permeabilized to 1-N-phenyl-naphthylamine than was its parent strain. These data were consistent with uptake of aminoglycosides across the E. coli outer membrane by the self-promoted uptake mechanism.
引用
收藏
页码:1309 / 1314
页数:6
相关论文
共 32 条
[21]   DIFFUSION OF AMINOGLYCOSIDE ANTIBIOTICS ACROSS THE OUTER-MEMBRANE OF ESCHERICHIA-COLI [J].
NAKAE, R ;
NAKAE, T .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1982, 22 (04) :554-559
[22]  
NICAS TI, 1980, J BACTERIOL, V143, P872, DOI 10.1128/JB.143.2.872-878.1980
[23]  
NIKAIDO H, 1985, MICROBIOL REV, V4, P1
[24]   LIPOPOLYSACCHARIDE-FREE ESCHERICHIA-COLI OMPF AND PSEUDOMONAS-AERUGINOSA PROTEIN-P PORINS ARE FUNCTIONALLY ACTIVE IN LIPID BILAYER-MEMBRANES [J].
PARR, TR ;
POOLE, K ;
CROCKFORD, GWK ;
HANCOCK, REW .
JOURNAL OF BACTERIOLOGY, 1986, 165 (02) :523-526
[25]   DECREASED BINDING OF ANTIBIOTICS TO LIPOPOLYSACCHARIDES FROM POLYMYXIN-RESISTANT STRAINS OF ESCHERICHIA-COLI AND SALMONELLA-TYPHIMURIUM [J].
PETERSON, AA ;
FESIK, SW ;
MCGROARTY, EJ .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1987, 31 (02) :230-237
[26]   ENHANCED BINDING OF POLYCATIONIC ANTIBIOTICS TO LIPOPOLYSACCHARIDE FROM AN AMINOGLYCOSIDE-SUPERSUSCEPTIBLE, TOLA MUTANT STRAIN OF PSEUDOMONAS-AERUGINOSA [J].
RIVERA, M ;
HANCOCK, REW ;
SAWYER, JG ;
HAUG, A ;
MCGROARTY, EJ .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1988, 32 (05) :649-655
[27]   SELECTION OF MULTIPLE ANTIBIOTIC-RESISTANCE BY QUINOLONES, BETA-LACTAMS, AND AMINOGLYCOSIDES WITH SPECIAL REFERENCE TO CROSS-RESISTANCE BETWEEN UNRELATED DRUG CLASSES [J].
SANDERS, CC ;
SANDERS, WE ;
GOERING, RV ;
WERNER, V .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1984, 26 (06) :797-801
[28]   OUTER-MEMBRANE PERMEATION OF BETA-LACTAM ANTIBIOTICS IN ESCHERICHIA-COLI, PROTEUS-MIRABILIS, AND ENTEROBACTER-CLOACAE [J].
SAWAI, T ;
HIRUMA, R ;
KAWANA, N ;
KANEKO, M ;
TANIYASU, F ;
INAMI, A .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1982, 22 (04) :585-592
[29]   DECREASED BINDING OF POLYMYXIN BY POLYMYXIN-RESISTANT MUTANTS OF SALMONELLA-TYPHIMURIUM [J].
VAARA, M ;
VAARA, T ;
SARVAS, M .
JOURNAL OF BACTERIOLOGY, 1979, 139 (02) :664-667
[30]   OUTER-MEMBRANE PERMEABILITY BARRIER DISRUPTION BY POLYMYXIN IN POLYMYXIN-SUSCEPTIBLE AND POLYMYXIN-RESISTANT SALMONELLA-TYPHIMURIUM [J].
VAARA, M ;
VAARA, T .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1981, 19 (04) :578-583