CONTROLLED-RELEASE OF POLYPEPTIDES FROM POLYANHYDRIDES

被引：63

作者：

RON, E

TUREK, T

MATHIOWITZ, E

CHASIN, M

HAGEMAN, M

LANGER, R

机构：

[1] MIT,DEPT CHEM ENGN,CAMBRIDGE,MA 02138

[2] NOVA PHARMACEUT CORP,BALTIMORE,MD 21224

[3] UPJOHN CO,KALAMAZOO,MI 49001

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 09期

关键词：

BOVINE GROWTH HORMONE; DRUG DELIVERY SYSTEMS; CONTROLLED RELEASE; DEGRADABLE POLYMERS;

D O I：

10.1073/pnas.90.9.4176

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The effects of both polymer hydrophobicity and addition of stabilizers on the release and integrity of polymer-encapsulated proteins were studied. By using very hydrophobic poly[1,3-bis(p-carboxyhydroxy)hexane anhydride] with sucrose as an excipient, both recombinant bovine somatotropin and zinc insulin were released intact over 3 weeks. The released proteins appeared to maintain their integrity as judged by acidic reverse-phase HPLC, size-exclusion HPLC, radioimmunoassay, and conformation-sensitive immunoassays. Our results also suggest how polymer hydrophobicity can be used to enhance protein stability.

引用

页码：4176 / 4180

页数：5

共 29 条

[1] INCREASED THERMAL-STABILITY OF PROTEINS IN THE PRESENCE OF SUGARS AND POLYOLS [J].