CHARACTERIZATION OF THE NUCLEAR GENE ENCODING CHLOROPLAST RIBOSOMAL-PROTEIN S13 FROM ARABIDOPSIS-THALIANA

被引：10

作者：

KUMAR, R

DROUAUD, J

RAYNAL, M

SMALL, I

机构：

[1] INRA,GENET & AMELIORAT PLANTES STN,F-78026 VERSAILLES,FRANCE

[2] UNIV PERPIGNAN,PHYSIOL & BIOL MOLEC VEGETALE LAB,CNRS,F-66025 PERPIGNAN,FRANCE

来源：

CURRENT GENETICS | 1995年 / 28卷 / 04期

关键词：

ARABIDOPSIS THALIANA; RIBOSOMAL PROTEIN; CHLOROPLAST S13; MITOCHONDRIAL S13;

D O I：

10.1007/BF00326432

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have characterised a cDNA clone and a nuclear gene encoding the chloroplast 30 s ribosomal protein S13 from Arabidopsis thaliana. The identification is based on the high similarity of the predicted amino-acid sequence with eubacterial S13 protein sequences, and immunodetection of a 14.5-kDa chloroplast ribosomal polypeptide using antibodies raised against the polypeptide produced from part of the cDNA expressed in bacteria. The predicted amino-acid sequence contains an N-terminal extension which has several features characteristic of chloroplast transit peptides. Experiments suggest there is a single copy of this gene in A. thaliana and multiple copies in Brassica species. The origin of the mitochondrial S13 polypeptide in crucifers is also discussed.

引用

页码：346 / 352

页数：7