IDENTIFICATION AND CHARACTERIZATION OF PHASEOLIN POLYPEPTIDES IN A CRYSTALLINE PROTEIN ISOLATED FROM WHITE KIDNEY BEANS (PHASEOLUS-VULGARIS)

被引：16

作者：

ALLI, I ^{[1
]}

GIBBS, BF ^{[1
]}

OKONIEWSKA, MK ^{[1
]}

KONISHI, Y ^{[1
]}

DUMAS, F ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA, BIOTECHNOL RES INST, MONTREAL H4P 2R2, PQ, CANADA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1993年 / 41卷 / 11期

关键词：

D O I：

10.1021/jf00035a005

中图分类号：

S [农业科学];

学科分类号：

09 [农学];

摘要：

A crystalline food protein isolated from dried seeds of white kidney beans (Phaseolus vulgaris) was investigated for the presence and characteristics of phaseolin polypeptides. Reversed-phase high-performance liquid chromatography (RP-HPLC) of the crystalline protein gave five fractions, of which the first two eluting fractions (F1 and F2) were found to contain phaseolin polypeptides. Ion-spray mass spectrometry showed that the average molecular weights (MW) for F1 and F2 were 49 615 and 48 075. Amino acid composition and N-terminal sequence analysis indicated that the fractions F1 and F2, separated from the crystalline protein, contained polypeptides which were similar to those reported for alpha-type and beta-type phaseolin precursors, respectively.

引用

页码：1830 / 1834

页数：5

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