SINGLE-STEP PCR FOR DETECTION OF BRUCELLA SPP FROM BLOOD AND MILK OF INFECTED ANIMALS

被引：160

作者：

LEALKLEVEZAS, DS

MARTINEZVAZQUEZ, IO

LOPEZMERINO, A

MARTINEZSORIANO, JP

机构：

[1] CTR INVEST BIOMED NORESTE, INST MEXICANO SEGURO SOCIAL, MONTERREY 64720, NUEVO LEON, MEXICO

[2] UNIV AUTONOMA NUEVO LEON, FAC CIENCIAS BIOL, SAN NICOLAS DE GARZAS, NUEVO LEON, MEXICO

[3] INST POLITECN NACL, ESCUELA NACL CIENCIAS BIOL, MEXICO CITY 11340, DF, MEXICO

[4] INST NACL DIAGNOST & REFERENCIA EPIDEMIOL, MEXICO CITY 11340, DF, MEXICO

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 12期

关键词：

D O I：

10.1128/JCM.33.12.3087-3090.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A versatile method for the extraction of Brucella DNA and PCR are presented as reliable tools for the detection of Brucella spp. from body fluids of infected animals. Two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (omp-2) were designed to detect the pathogen from milk and/or blood of infected goats, bovines, and human patients. The sensitivity of our test and its ability to detect the pathogen in samples from the field reveal a promising advance in the diagnosis of brucellosis in animals and humans.

引用

页码：3087 / 3090

页数：4

共 30 条

[21]

MARTINEZSORIANO JP, 1993, BIOQUIMIA, V18, P10

[22]

MARTINEZSORIANO JP, UNPUB

[23]

MARTINEZVAZQUEZ IO, 1995, THESIS U AUTONOMA NU

[24]

Morgan W. J. Brinley, 1960, Research in Veterinary Science, V1, P53

[25]

Nicoletti P, 1980, Adv Vet Sci Comp Med, V24, P69

[26] ANTIGENIC RELATIONSHIPS OF THE LIPOPOLYSACCHARIDES OF ESCHERICHIA-HERMANNII STRAINS WITH THOSE OF ESCHERICHIA-COLI O157-H7, BRUCELLA-MELITENSIS, AND BRUCELLA-ABORTUS [J].

PERRY, MB ;

BUNDLE, DR .

INFECTION AND IMMUNITY, 1990, 58 (05) :1391-1395

[27]

RUIZCASTANEDA M, 1986, BRUCELOSIS TERCERA

[28] PRIMER-DIRECTED ENZYMATIC AMPLIFICATION OF DNA WITH A THERMOSTABLE DNA-POLYMERASE [J].

SAIKI, RK ;

GELFAND, DH ;

STOFFEL, S ;

SCHARF, SJ ;

HIGUCHI, R ;

HORN, GT ;

MULLIS, KB ;

ERLICH, HA .

SCIENCE, 1988, 239 (4839) :487-491

[29]

Sambrook J., 1989, MOL CLONING LAB MANU

[30]

1994, SALUD ANIMAL MEXICO

← 1 2 3 →