LOSS OF CARDIAC CONTRACTILITY AND SEVERE MORPHOLOGIC CHANGES BY ACUTELY LOWERING THE PH OF THE PERFUSION MEDIUM - PROTECTION BY FATTY-ACIDS

被引:14
作者
HULSMANN, WC
DEWIT, LEA
SCHNEYDENBERG, C
VERKLEIJ, AJ
机构
[1] ERASMUS UNIV, DEPT BIOCHEM, 3000 DR ROTTERDAM, NETHERLANDS
[2] STATE UNIV UTRECHT, DEPT MOLEC CELL BIOL, UTRECHT, NETHERLANDS
关键词
Acidosis; Cardiac structure-function; Fatty acid; Ischemia;
D O I
10.1016/0304-4165(90)90016-P
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
When the pH of the perfusion medium of rat Langendorff heart, paced at a rate of 300 beats/min, is abruptly lowered from pH 7.5 to 7.0, the hearts stop beating within 6 min more than half of the cases. Reperfusion with pH 7.5 medium after 10 min pH 7.0 perfusion does not cause contractility to resume within 5 min. The causative factor is intracellular acidosis, resulting in severe morphological alterations of plasma membrane and mitochondria. It is probably initiated by the loss of membrane-bound calcium. Oleate, complexed with albumin included in the perfusion media, protects the hearts. This may be explained by maintenance of capillary flow and limitation of cellular acidosis. © 1990.
引用
收藏
页码:214 / 218
页数:5
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