STUDIES ON CHARACTERIZATION OF THERMOSTABLE ANTIGENS OF ADRENALS AND MUSCLE TISSUES OF MEAT ANIMALS

被引:10
作者
BHILEGAONKAR, KN
SHERIKAR, AT
KHOT, JB
KARKARE, UD
机构
[1] Department of Food Hygiene, Veterinary Public Health, Bombay Veterinary College, Bombay, 400012, Parel
关键词
adrenal; meat speciation; muscle; Thermostable antigen; troponin T;
D O I
10.1002/jsfa.2740510411
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The characteristics of heat‐stable, ethanol‐precipitable antigens (BE) of adrenals and muscle tissues of cattle, buffalo, sheep, goat and pig were studied. The antigens were subjected to protein analysis. The distribution patterns of the antigens employing sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE) as well as polyacrylarnide gel isoelectric focusing revealed that the heat‐stable antigens of a dreds contained only one component corresponding to troponin T, whereas muscle BE showed associated low molecular weight components along with troponin T as major component. Negative results of thymol‐H2SO4 staining of SDS‐PAGE gels indicated that the thermostable antigens did not belong to the mucoprotein class. Copyright © 1990 John Wiley & Sons, Ltd
引用
收藏
页码:545 / 553
页数:9
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