THE LARGE ISOFORM OF MYELIN-ASSOCIATED GLYCOPROTEIN IS SCARCELY EXPRESSED IN THE QUAKING MOUSE-BRAIN

被引：34

作者：

FUJITA, N ^{[1
]}

SATO, S ^{[1
]}

ISHIGURO, H ^{[1
]}

INUZUKA, T ^{[1
]}

BABA, H ^{[1
]}

KURIHARA, T ^{[1
]}

TAKAHASHI, Y ^{[1
]}

MIYATAKE, T ^{[1
]}

机构：

[1] NIIGATA UNIV,DEPT NEUROPHARMACOL,NIIGATA 951,JAPAN

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 55卷 / 03期

关键词：

Alternative splicing; mRNA; Myelin‐associated glycoprotein; Quaking mouse;

D O I：

10.1111/j.1471-4159.1990.tb04596.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: Two polypeptide isoforms of myelin‐associated glycoprotein (MAG) with molecular masses of 72 and 67 kDa are produced by alternative splicing of the exon 12 portion. Our previous work has demonstrated that in the quaking mouse brain this alternative splicing is lacking and that the mRNA coding the large MAG isoform (L‐MAG) is scarcely expressed, whereas that of small MAG isoform (S‐MAG) is overexpressed. In the present study, we prepared antisera specific to the S‐MAG and L‐MAG amino acid residues, respectively. Immunoblots showed that the L‐MAG band was scarcely detectable in the quaking mouse brain, whereas the S‐MAG band had an apparently higher molecular mass than in the normal control. Our immunohistochemical study also showed that L‐MAG was scarcely stained in the quaking mouse brain. These results seemed to reflect a reduction in content of L‐MAG mRNA and abnormal glycosylation in the quaking mouse brain. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1056 / 1059

页数：4

共 24 条

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