Determination of penicillins in animal tissues at trace residue concentrations .1. Determination of benzylpenicillin in milk by reversed-phase liquid chromatography with solid phase extraction and liquid chromatographic fractionation clean-up

被引：15

作者：

Tarbin, J ^{[1
]}

Farrington, WH ^{[1
]}

Shearer, G ^{[1
]}

机构：

[1] MAFF,CSL FOOD SCI LAB,NORWICH NR4 7UQ,NORFOLK,ENGLAND

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 318卷 / 01期

关键词：

liquid chromatography; penicillins; animal tissue; benzylpenicillin; milk;

D O I：

10.1016/0003-2670(95)00320-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A liquid chromatographic (LC) method for the determination of benzylpenicillin (penicillin G) in milk has been developed. Milk was decreamed by centrifugation and deproteinised using sulphuric acid/sodium tungstate. Initial clean-up was achieved using C-18 solid phase extraction (SPE). The SPE eluate was cleaned-up by reversed-phase LC fractionation at neutral pH. Final determination was by reversed-phase LC at acidic pH with post-column derivatisation with imidazole/mercury(II) chloride reagent followed by UV absorbance determination at 320 Mm. Average recoveries at 2 and 10 mu g kg(-1) were 70-73%. The lower limit of quantification was 2 mu g kg(-1).

引用

页码：95 / 101

页数：7