CALCIUM-CHANNEL BLOCKERS AND PROSTAGLANDIN GENERATION BY GASTRIC SURFACE EPITHELIAL-CELLS

被引：6

作者：

FUKUDA, T ^{[1
]}

ARAKAWA, T ^{[1
]}

MACH, T ^{[1
]}

DURBIN, T ^{[1
]}

TARNAWSKI, A ^{[1
]}

机构：

[1] UNIV CALIF IRVINE, DEPT MED, IRVINE, CA 92717 USA

来源：

PROSTAGLANDINS & OTHER LIPID MEDIATORS | 1991年 / 42卷 / 06期

关键词：

D O I：

10.1016/0090-6980(91)90020-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the effects of calcium channel blockers on generation of prostaglandin (PG) E2 and 6-keto PGF1-alpha by gastric mucosal surface epithelium. Surface epithelial cells (SEC) isolated from rat gastric mucosa were incubated with either verapamil (1 or 10-mu-g/ml), diltiazem (2.5 or 25-mu-g/ml) or nifedipine (2.5 or 25-mu-g/ml) for 30 min at 37-degrees-C in calcium containing or calcium-free medium. Verapamil (both doses) significantly increased PGE2 and 6-keto PGF1-alpha generation by surface epithelial cells but only in calcium containing medium. Diltiazem did not affect PG generation in calcium containing nor calcium-free medium. Nifedipine 25-mu-g/ml decreased PGE2 but increased 6-keto PGF1-alpha generation. The inhibitory effect of nifedipine on PGE2 generation was abolished in calcium-free medium, while the calmodulin antagonist did not affect verapamil-induced increase in PG generation.

引用

页码：587 / 597

页数：11