METABOLIC CONVERSION OF 24-EPI-BRASSINOLIDE INTO PENTAHYDROXYLATED BRASSINOSTEROID GLUCOSIDES IN TOMATO CELL-CULTURES

被引：36

作者：

HAI, T

SCHNEIDER, B

ADAM, G

机构：

[1] Institut für Pflanzenbiochemie, 06120 Halle

来源：

PHYTOCHEMISTRY | 1995年 / 40卷 / 02期

关键词：

LYCOPERSICON ESCULENTUM; SOLANACEAE; BRASSINOSTEROIDS; CELL SUSPENSION CULTURES; CYTOCHROME P-450 INHIBITORS; GLUCOSIDES; GLUCOSYLTRANSFERASE; HYDROXYLATION; METABOLISM; RICE LAMINA INCLINATION TEST;

D O I：

10.1016/0031-9422(95)00224-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two isomeric metabolites, 25-beta-D-glucopyranosyloxy-24-epi-brassinolide and 26-beta-D-glucopyranosyloxy-24-epi-brassinolide, have been formed in cell suspension cultures of Lycopersicon esculentum from exogenously applied 24-epi-brassinolide. The two-step metabolic process involved hydroxylation of the side-chain at C-25 and C-26, respectively, followed by glucosidation of the newly formed hydroxyl groups. The ratio between both metabolites was significantly altered by in vivo treatment of the cell cultures with various cytochrome P-450-specific inhibitors, indicating the involvement of two different enzyme systems. Biosynthetically prepared 25-hydroxy-24-epibrassinolide, reapplied to cell cultures, was exclusively glucosylated at the 25-hydroxyl group, strongly suggesting regiospecificity of the corresponding glucosyltransferase.

引用

页码：443 / 448

页数：6

共 23 条

[1] BRASSINOSTEROIDS - A NEW GROUP OF PHYTOHORMONES
ADAM, G
PETZOLD, U
[J]. NATURWISSENSCHAFTEN, 1994, 81 (05) : 210 - 217
[2] INFLUENCE OF MONOOXYGENASE INHIBITORS ON THE METABOLISM OF THE HERBICIDES CHLORTOLURON AND METOLACHLOR IN CELL-SUSPENSION CULTURES
COLE, DJ
OWEN, WJ
[J]. PLANT SCIENCE, 1987, 50 (01) : 13 - 20
[3] DURST F, 1991, FRONTIERS BIOTRANSFO, V4, P191
[4] GERARDY R, 1993, PHYTOCHEMISTRY, V32, P79, DOI 10.1016/0031-9422(92)80111-Q
[5] GRAEBE JE, 1984, BER DEUT BOT GES, V97, P67
[6] HAI TNM, UNPUB
[7] GLYCYRRHETINIC ACID 24-HYDROXYLASE ACTIVITY IN MICROSOMES OF CULTURED LICORICE CELLS
HAYASHI, H
HANAOKA, S
TANAKA, S
FUKUI, H
TABATA, M
[J]. PHYTOCHEMISTRY, 1993, 34 (05) : 1303 - 1307
[8] STEREOCHEMISTRY AND PHYSIOLOGICAL SIGNIFICANCE OF HYDROXYLATION OF VITAMIN-D SIDE-CHAIN
IKEKAWA, N
[J]. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1993, 3 (09) : 1789 - 1794
[9] IMAI S, 1968, TETRAHEDRON LETT, P3887
[10] Kappler C., 1989, P161

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