GLYCYRRHETINIC ACID 24-HYDROXYLASE ACTIVITY IN MICROSOMES OF CULTURED LICORICE CELLS

被引：8

作者：

HAYASHI, H

HANAOKA, S

TANAKA, S

FUKUI, H

TABATA, M

机构：

[1] Faculty of Pharmaceutical Sciences, Kyoto University, Yoshida, Kyoto

来源：

PHYTOCHEMISTRY | 1993年 / 34卷 / 05期

关键词：

GLYCYRRHIZA-GLABRA; LEGUMINOSAE; 18-BETA-GLYCYRRHETINIC ACID; CYTOCHROME P-450; HYDROXYLATION; GLUCURONYLATION;

D O I：

10.1016/0031-9422(91)80020-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

18beta-Glycyrrhetinic acid 24-hydroxylase activity was detected in the microsomal fraction prepared from Glycyrrhiza glabra cell suspension cultures. The enzymatic activity required both NADPH and O2. The participation of cytochrome P-450 in the reaction was demonstrated by inhibitor experiments including the light reversibility of CO inhibition. The 24-hydroxylation of 18beta-glycyrrhetinic acid was shown to be prerequisite to forming its 3-O-glycoside by glucuronylation which is catalysed by a glucuronyltransferase localized also in the microsomal fraction of cultured licorice cells.

引用

页码：1303 / 1307

页数：5

共 19 条

[1] 2 METHYLENEDIOXY BRIDGE FORMING CYTOCHROME-P-450 DEPENDENT ENZYMES ARE INVOLVED IN (S)-STYLOPINE BIOSYNTHESIS [J].