STABLE ISOTOPE-LABELED VITAMIN-D, METABOLITES AND CHEMICAL ANALOGS - SYNTHESIS AND USE IN MASS-SPECTROMETRIC STUDIES

被引：19

作者：

COLDWELL, RD ^{[1
]}

TRAFFORD, DJH ^{[1
]}

VARLEY, MJ ^{[1
]}

KIRK, DN ^{[1
]}

MAKIN, HLJ ^{[1
]}

机构：

[1] UNIV LONDON LONDON HOSP,COLL MED,DEPT CHEM PATHOL,TURNER ST,LONDON E1 2AD,ENGLAND

来源：

STEROIDS | 1990年 / 55卷 / 10期

关键词：

STEROIDS; VITAMIN-D METABOLITES; DEUTERIUM LABELING; GAS CHROMATOGRAPHY; MASS SPECTROMETRY;

D O I：

10.1016/0039-128X(90)90010-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods for the measurement of vitamin D and its metabolites using stable isotope-labeled internal standards and mass spectrometry are reviewed. The synthesis of both labeled and unlabeled standards is illustrated, and details of the synthesis of (26,26,27,27,27-H-2(5))-25,26-dihydroxyvitamin D3 and (28,28,28-H-2(3))-24,25-dihydroxyvitamin D2 are given. The use of in vitro biologic systems for the production of further metabolites of deuterated 25-hydroxyvitamin D3 is discussed. Use of deuterated 25-hydroxydihydrotachysterol 3 as a substrate in the isolated perfused rat kidney has provided valuable data for the assignment of structure to a number of metabolites of 25-hydroxydihydrotachysterol 3 formed in this system.

引用

页码：418 / 432

页数：15

共 51 条

[1]

Porteous, Coldwell, Trafford, Makin, Recent developments in the measurement of vitamin D and its metabolites in human body fluids, J Steroid Biochem, 28, pp. 785-801, (1987)

[2]

Jongen, VanGinkel, van der Vijgh, Kuiper, Netelenbos, Lips, An international comparison of vitamin D metabolite measurements, Clin Chem, 30, pp. 399-403, (1984)

[3]

MayerE, Schmidt-Gayk, Interlaboratory comparison of 25-hydroxyvitamin D determination, Clin Chem, 30, pp. 1199-1204, (1984)

[4]

Lawson, Calam, Colinet, Certification of cortisol in two lyophilised serum samples, BCR no. 192 and 193, Report EUR 9661 EN, (1985)

[5]

Coldwell, Porteous, Trafford, Makin, Gas chromatography-mass spectrometry and the measurement of vitamin D metabolites in human serum or plasma, Steroids, 49, pp. 155-196, (1987)

[6]

Makin, Trafford, Measurement of vitamin D and its metabolites by gas chromatography-mass spectrometry, Vitamin D: Basic and Clinical Aspects, pp. 497-521, (1984)

[7]

Seamark, Trafford, Makin, The estimation of vitamin D and some metabolites in human plasma by mass fragmentography, Clin Chim Acta, 106, pp. 51-62, (1980)

[8]

Bjorkhem, Holmberg, Mass fragmentographic assay of 25-hydroxyvitamin D3, Methods Enzymol, 67, pp. 385-393, (1980)

[9]

Bjorkhem, Holmberg, A novel specific assay of 25-hydroxyvitamin D3, Clinica Chimica Acta, 68, pp. 215-221, (1976)

[10]

Bjorkhem, Holmberg, Kristiansen, Pedersen, Assay of 1,25-dihydroxyvitamin D3 by isotope dilution-mass fragmentography, Clin Chem, 25, pp. 584-588, (1979)

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