STABLE ISOTOPE-LABELED VITAMIN-D, METABOLITES AND CHEMICAL ANALOGS - SYNTHESIS AND USE IN MASS-SPECTROMETRIC STUDIES

被引：19

作者：

COLDWELL, RD ^{[1
]}

TRAFFORD, DJH ^{[1
]}

VARLEY, MJ ^{[1
]}

KIRK, DN ^{[1
]}

MAKIN, HLJ ^{[1
]}

机构：

[1] UNIV LONDON LONDON HOSP,COLL MED,DEPT CHEM PATHOL,TURNER ST,LONDON E1 2AD,ENGLAND

来源：

STEROIDS | 1990年 / 55卷 / 10期

关键词：

STEROIDS; VITAMIN-D METABOLITES; DEUTERIUM LABELING; GAS CHROMATOGRAPHY; MASS SPECTROMETRY;

D O I：

10.1016/0039-128X(90)90010-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methods for the measurement of vitamin D and its metabolites using stable isotope-labeled internal standards and mass spectrometry are reviewed. The synthesis of both labeled and unlabeled standards is illustrated, and details of the synthesis of (26,26,27,27,27-H-2(5))-25,26-dihydroxyvitamin D3 and (28,28,28-H-2(3))-24,25-dihydroxyvitamin D2 are given. The use of in vitro biologic systems for the production of further metabolites of deuterated 25-hydroxyvitamin D3 is discussed. Use of deuterated 25-hydroxydihydrotachysterol 3 as a substrate in the isolated perfused rat kidney has provided valuable data for the assignment of structure to a number of metabolites of 25-hydroxydihydrotachysterol 3 formed in this system.

引用

页码：418 / 432

页数：15