SIMULTANEOUS, RAPID, SPECTROPHOTOMETRIC DETERMINATION OF TOTAL STARCH, AMYLOSE AND AMYLOPECTIN

被引：153

作者：

JARVIS, CE ^{[1
]}

WALKER, JRL ^{[1
]}

机构：

[1] UNIV CANTERBURY,DEPT PLANT & MICROBIAL SCI,CHRISTCHURCH,NEW ZEALAND

来源：

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 1993年 / 63卷 / 01期

关键词：

AMYLOSE; AMYLOPECTIN; STARCH; SPECTROPHOTOMETRIC ASSAY;

D O I：

10.1002/jsfa.2740630109

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A novel spectrophotometric method for the simultaneous measurement of total starch, amylose and amylopectin in lipid-free samples has been developed using a diode-array spectrophotometer. It involves measurement of the spectrum of the amylose/amylopectin-iodide complexes at six wavelengths and calculation of amylose and amylopectin concentrations using multi-component analysis and a spreadsheet computer program. This method appears to offer some advantages to previous 'iodine-blue colour' assays for the measurement of amylose.

引用

页码：53 / 57

页数：5

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