SEQUENCE-ANALYSIS OF MOUSE CDNAS ENCODING RIBOSOMAL-PROTEINS L12 AND L18

被引：4

作者：

HOU, EW ^{[1
]}

LI, SSL ^{[1
]}

机构：

[1] NIEHS,GENET LAB,MAIL DROP D3-05,POB 12233,RES TRIANGLE PK,NC 27709

来源：

GENE | 1993年 / 130卷 / 02期

关键词：

RIBOSOMES; RAT; XENOPUS; GENOMIC ORGANIZATION; PSEUDOGENES;

D O I：

10.1016/0378-1119(93)90433-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Mouse cDNAs encoding ribosomal proteins (r-proteins), L12 and L18, were isolated and their sequences determined. The L12 cDNA was found to contain 639 bp, including a coding sequence of 498 nucleotides (nt), 5' (78 nt) and 3' (45 nt) untranslated regions (UTRs), and a poly(A) tail of 18 nt. The L18 cDNA was shown to consist of 648 bp, including a coding sequence of 567 nt, 5' (26 nt) and 3' (39 nt) UTRs, and a poly(A) tail of 16 nt. The nt sequences of the protein-coding region from the mouse L12 and L18 cDNAs were found to exhibit 96% and 92% identity, respectively, with those of the rat. With the use of mouse L12 and L18 cDNA probes, multiple (at least 10) copies of the L12 and L18 gene families were shown to be present in the mouse and rat genomes. However, there was no sequence heterogeneity detected among seven L18 cDNA clones, indicating that only one copy of the L18 gene-related sequences is functional, and the other copies are presumably nonfunctional pseudogenes. The complete amino acid (aa) sequences of the mouse r-proteins, L12 and L18, were deduced from the nt sequences of their cDNA clones. L12 has 165 aa and a M(r) of 17790, while L18 has 188 aa and a M(r) of 21 570. The aa sequences of the mouse r-proteins, L12 and L18, exhibit 98% and 94% identity, respectively, to those of rat.

引用

页码：287 / 290

页数：4

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