INCREASE IN ORNITHINE DECARBOXYLASE ACTIVITY CAUSED BY HEPATOCYTE GROWTH-FACTOR IN PRIMARY CULTURED ADULT-RAT HEPATOCYTES

被引：18

作者：

HIGAKI, I ^{[1
]}

MATSUIYUASA, I ^{[1
]}

TERAKURA, M ^{[1
]}

KINOSHITA, H ^{[1
]}

OTANI, S ^{[1
]}

机构：

[1] OSAKA CITY UNIV,SCH MED,DEPT SURG 2,OSAKA 545,JAPAN

来源：

HEPATOLOGY | 1993年 / 17卷 / 01期

关键词：

D O I：

10.1002/hep.1840170118

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The effect of hepatocyte growth factor on ornithine decarboxylase activity was studied in primary cultured adult rat hepatocytes. Ornithine decarboxylase activity was increased 3 hr after the addition of hepatocyte growth factor and remained at a high level until 12 hr; thereafter it decreased, and it returned to the control level by 24 hr. Enzyme activity began to increase with 1 ng/ml hepatocyte growth factor and reached its maximum with 5 ng/ml hepatocyte growth factor. When insulin or epidermal growth factor was added with hepatocyte growth factor, enzyme activity was further stimulated. The level of ornithine decarboxylase messenger RNA did not increase with addition of hepatocyte growth factor. The half-time of ornithine decarboxylase activity was prolonged about twofold by hepatocyte growth factor treatment. These results suggest that hepatocyte growth factor treatment of cells enhanced ornithine decarboxylase activity posttranslationally

引用

页码：99 / 102

页数：4

共 15 条

[11] EFFECT OF ALPHA-DIFLUOROMETHYLORNITHINE ON POLYAMINE AND DNA-SYNTHESIS IN REGENERATING RAT-LIVER - REVERSAL OF INHIBITION OF DNA-SYNTHESIS BY PUTRESCINE [J].