CONSTRUCTION AND EXPRESSION OF THE COMPLETE CLOSTRIDIUM-DIFFICILE TOXIN-A GENE IN ESCHERICHIA-COLI

被引：27

作者：

PHELPS, CJ

LYERLY, DL

JOHNSON, JL

WILKINS, TD

机构：

来源：

INFECTION AND IMMUNITY | 1991年 / 59卷 / 01期

关键词：

D O I：

10.1128/IAI.59.1.150-153.1991

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Cloned fragments constituting the 8.1-kb toxin A gene of Clostridium difficile were used to reconstruct the intact gene. The recombinant toxin expressed in Escherichia coli was cytotoxic, enterotoxic, and lethal. In addition, toxic lysate caused hemagglutination of rabbit erythrocytes. The toxic activities were inhibited by antibody specific for toxin A. Our findings demonstrate that the biological activities exhibited by native toxin A are functions of a single protein encoded by the 8.1-kb toxin A gene, independent of any other C. difficile gene products.

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页码：150 / 153

页数：4

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