DEVELOPMENT AND OPTIMIZATION OF AN IMMUNOAFFINITY-BASED SOLID-PHASE EXTRACTION FOR CHLORTOLURON

被引:40
作者
SHAHTAHERI, SJ [1 ]
KATMEH, MF [1 ]
KWASOWSKI, P [1 ]
STEVENSON, D [1 ]
机构
[1] UNIV SURREY,ROBENS INST,GUILDFORD GU2 5XH,SURREY,ENGLAND
关键词
D O I
10.1016/0021-9673(94)01088-V
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The determination of trace organics such as pesticides in biological and environmental samples require rapid, easy-to-use, reliable sample preparation procedures. Solid-phase extraction using silica or bonded silicas has proven useful for broad-range screening. We have used antisera to chlortoluron immobilised onto silica as a ''tailor-made'' solid-phase extraction system. The chlortoluron can be selectively retained and eluted using a simple phosphate buffered saline/ethanol mixture. Preliminary studies have demonstrated that the immune column has a high volume breakthrough (at least 11 of water) and can retain up to 500 ng of chlortoluron. Quantitative recovery from tap water, river water, drinking water, plasma and urine is achieved along with an HPLC trace free from co-eluting compounds at the chlortoluron retention time.
引用
收藏
页码:131 / 136
页数:6
相关论文
共 8 条
[1]  
AHERNE G W, 1987, Analytical Proceedings, V24, P140
[3]   ONLINE SAMPLE TREATMENT FOR OR VIA COLUMN LIQUID-CHROMATOGRAPHY [J].
BRINKMAN, UAT .
JOURNAL OF CHROMATOGRAPHY A, 1994, 665 (02) :217-231
[4]   ONLINE IMMUNOAFFINITY SAMPLE PRETREATMENT FOR COLUMN LIQUID-CHROMATOGRAPHY - EVALUATION OF DESORPTION TECHNIQUES AND OPERATING-CONDITIONS USING AN ANTIESTROGEN IMMUNO-PRECOLUMN AS A MODEL SYSTEM [J].
FARJAM, A ;
BRUGMAN, AE ;
LINGEMAN, H ;
THBRINKMAN, UA .
ANALYST, 1991, 116 (09) :891-896
[5]   IMMUNOAFFINITY PRECOLUMN FOR SELECTIVE SAMPLE PRETREATMENT IN COLUMN LIQUID-CHROMATOGRAPHY - IMMUNOSELECTIVE DESORPTION [J].
FARJAM, A ;
BRUGMAN, AE ;
SOLDAAT, A ;
TIMMERMAN, P ;
LINGEMAN, H ;
DEJONG, GJ ;
FREI, RW ;
BRINKMAN, UAT .
CHROMATOGRAPHIA, 1991, 31 (9-10) :469-477
[6]   DETERMINATION OF CARBOFURAN BY ONLINE IMMUNOAFFINITY CHROMATOGRAPHY WITH COUPLED-COLUMN LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY [J].
RULE, GS ;
MORDEHAI, AV ;
HENION, J .
ANALYTICAL CHEMISTRY, 1994, 66 (02) :230-235
[7]   AUTOMATED LIQUID-CHROMATOGRAPHIC DETERMINATION OF OCHRATOXIN-A IN CEREALS AND ANIMAL PRODUCTS USING IMMUNOAFFINITY COLUMN CLEANUP [J].
SHARMAN, M ;
MACDONALD, S ;
GILBERT, J .
JOURNAL OF CHROMATOGRAPHY, 1992, 603 (1-2) :285-289
[8]   DETERMINATION OF CHLORAMPHENICOL IN SWINE MUSCLE-TISSUE USING A MONOCLONAL ANTIBODY-MEDIATED CLEANUP PROCEDURE [J].
VANDEWATER, C ;
HAAGSMA, N .
JOURNAL OF CHROMATOGRAPHY, 1987, 411 :415-421