PURIFICATION AND CDNA SEQUENCE OF AN INDUCIBLE NITRIC-OXIDE SYNTHASE FROM A HUMAN TUMOR-CELL LINE

被引:211
作者
SHERMAN, PA [1 ]
LAUBACH, VE [1 ]
REEP, BR [1 ]
WOOD, ER [1 ]
机构
[1] BURROUGHS WELLCOME CO,DIV CELL BIOL,RES TRIANGLE PK,NC 27709
关键词
D O I
10.1021/bi00094a017
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A combination of cytokines induced the expression of nitric oxide synthase (NOS) in a human colorectal adenocarcinoma cell line, DLD-1. We have purified the enzyme and examined some of its biochemical properties. An antiserum to an inducible NOS from murine macrophages cross-reacted with the DLD-1 NOS. The purified human and murine enzymes displayed a similar lack of dependence on exogenous calcium and calmodulin for activity, which contrasts with the requirement for calcium and calmodulin of purified brain and endothelial isoforms of NOS. We have also isolated a cDNA for a cytokine-induced NOS from DLD-1 cells. Sequence analysis of this cDNA and NOS cDNAs from human liver, smooth muscle, and macrophages suggests that, at the genetic level, there is a single isoform of human-inducible NOS.
引用
收藏
页码:11600 / 11605
页数:6
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