PURIFICATION AND CHARACTERIZATION OF LIPOPOLYSACCHARIDE-BINDING PROTEIN FROM HEMOLYMPH OF AMERICAN COCKROACH PERIPLANETA-AMERICANA

被引：62

作者：

JOMORI, T ^{[1
]}

KUBO, T ^{[1
]}

NATORI, S ^{[1
]}

机构：

[1] UNIV TOKYO,FAC PHARMACEUT SCI,BUNKYO KU,TOKYO 113,JAPAN

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 190卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb15565.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A protein having affinity to lipopolysaccharide of Escherichia coli K12 was purified to homogeneity from the hemolymph of Periplaneta americana. This protein, with an average molecular mass of 450 kDa, was a homooligomer of a 28‐kDa subunit protein. Comparative studies using lipopolysaccharide molecules of E. coli and Salmonella minnesota suggested that this protein recognizes and binds to a specific carbohydrate structure of E. coli lipopolysaccharide. Ca2+ was required for this protein to bind to lipopolysaccharide, but other divalent cations could not replace Ca2+. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：201 / 206

页数：6

共 42 条

[1]

AKETAGAWA J, 1986, J BIOL CHEM, V261, P7357

[2] BIOCHEMICAL STUDIES ON LIPOPOLYSACCHARIDES OF SALMONELLA R MUTANTS .3. LINKAGE OF HEPTOSE UNITS [J].

DROGE, W ;

LUDERITZ, O ;

WESTPHAL, O .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1968, 4 (01) :126-&

[3]

ELSBACH P, 1979, J BIOL CHEM, V254, P1000

[4] ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE [J].

FAIRBANKS, G ;

STECK, TL ;

WALLACH, DFH .

BIOCHEMISTRY, 1971, 10 (13) :2606-+

[5] SUBCELLULAR LOCATION AND PROPERTIES OF BACTERICIDAL FACTORS FROM HUMAN-NEUTROPHILS [J].

GABAY, JE ;

HEIPLE, JM ;

COHN, ZA ;

NATHAN, CF .

JOURNAL OF EXPERIMENTAL MEDICINE, 1986, 164 (05) :1407-1421

[6] A NEW METHOD FOR EXTRACTION OF R-LIPOPOLYSACCHARIDES [J].

GALANOS, C ;

LUDERITZ, O ;

WESTPHAL, O .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1969, 9 (02) :245-&

[7] PRECIPITATION OF LIPOPOLYSACCHARIDES FROM ROUGH MUTANTS OF SALMONELLA-TYPHIMURIUM BY AN A-HEMAGGLUTININ FROM HELIX-POMATIA [J].

HAMMARSTROM, S ;

ROBERTSSON, ES ;

LINDBERG, AA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1972, 25 (02) :274-+

[8] PURIFICATION AND SOME PROPERTIES OF A LECTIN FROM THE HEMOLYMPH OF PERIPLANETA-AMERICANA (AMERICAN COCKROACH) [J].

KUBO, T ;

NATORI, S .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 168 (01) :75-82

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10]

LEVIN J, 1964, B JOHNS HOPKINS HOSP, V115, P265

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