THE SACCHAROMYCES-CEREVISIAE HIS3 AND LYS2 GENES COMPLEMENT THE SCHIZOSACCHAROMYCES-POMBE HIS5-303 AND LYS1-131 MUTATIONS, RESPECTIVELY - NEW SELECTABLE MARKERS AND NEW MULTIPURPOSE MULTICOPY SHUTTLE VECTORS, PSP3 AND PSP4

被引：12

作者：

COTTAREL, G ^{[1
]}

机构：

[1] COLD SPRING HARBOR LAB,HOWARD HUGHES MED INST,COLD SPRING HARBOR,NY 11724

来源：

CURRENT GENETICS | 1995年 / 28卷 / 04期

关键词：

AUTONOMOUSLY REPLICATING SEQUENCE; AUXOTROPHY; SCHIZOSACCHAROMYCES POMBE; SACCHAROMYCES CEREVISIAE; CLONING VECTOR; SELECTABLE MARKER; HIS/HIS; LYS/LYS;

D O I：

10.1007/BF00326437

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Three new S. pombe plasmids are described. Plasmids pSP3 and pSP4 are two Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae HIS3 or LYS2 genes as selectable markers. They complement the S. pombe his5-303 or lys1-131 mutations, respectively. Plasmid pSPars1 is a vector carrying the S. pombe ars1 and a unique NdeI site which allows the introduction of any selectable marker therefore bringing a unified vector backbone for the construction of new S. pombe/S. cerevisiae/E. coli shuttle vectors. These plasmids permit classical molecular genetic techniques to be performed directly.

引用

页码：380 / 383

页数：4

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