Direct database searching with MALDI-PSD spectra of peptides

被引：77

作者：

Griffin, PR

MacCoss, MJ

Eng, JK

Blevins, RA

Aaronson, JS

Yates, JR

机构：

[1] MERCK RES LABS, DEPT BIOINFORMAT, RAHWAY, NJ 07065 USA

[2] UNIV WASHINGTON, DEPT MOLEC BIOTECHNOL, SEATTLE, WA 98195 USA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1995年 / 9卷 / 15期

关键词：

D O I：

10.1002/rcm.1290091515

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The analysis of matrix-assisted laser desorption ionization post-source decay (MALDI-PSD) mass spectra of peptides by using the cross-correlation method for database searching is illustrated. MALDI-PSD mass spectra are shown to contain sufficient fragmentation information to uniquely identify the correct amino acid sequence from large protein databases (similar to 160 000 entries), A search employing the MALDI-PSD mass spectrum of a phosphorylated peptide that correctly identifies the amino acid sequence and the site of phosphorylation is also illustrated.

引用

页码：1546 / 1551

页数：6

共 20 条

[1] MASS-SPECTROMETRY OF PEPTIDES AND PROTEINS
BIEMANN, K
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1992, 61 : 977 - 1010
[2] BIEMANN K, 1990, METHODS ENZYMOLOGY
[3] COVEY T, 1991, ADV LIF SCI, P249
[4] IDENTIFICATION OF PHOSPHORYLATED PEPTIDES FROM COMPLEX-MIXTURES USING NEGATIVE-ION ORIFICE-POTENTIAL STEPPING AND CAPILLARY LIQUID-CHROMATOGRAPHY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY
DING, JM
BURKHART, W
KASSEL, DB
[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1994, 8 (01) : 94 - 98
[5] A PROTEIN SEQUENATOR
EDMAN, P
BEGG, G
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1967, 1 (01): : 80 - &
[6] AN APPROACH TO CORRELATE TANDEM MASS-SPECTRAL DATA OF PEPTIDES WITH AMINO-ACID-SEQUENCES IN A PROTEIN DATABASE
ENG, JK
MCCORMACK, AL
YATES, JR
[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (11) : 976 - 989
[7] SEQUENCING ELECTROBLOTTED PROTEINS BY TANDEM MASS-SPECTROMETRY
FABRIS, D
VESTLING, MM
CORDERO, MM
DOROSHENKO, VM
COTTER, RJ
FENSELAU, C
[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1995, 9 (11) : 1051 - 1055
[8] LIQUID SECONDARY-ION MASS-SPECTROMETRY OF PHOSPHORYLATED AND SULFATED PEPTIDES AND PROTEINS
GIBSON, BW
COHEN, P
[J]. METHODS IN ENZYMOLOGY, 1990, 193 : 480 - 501
[9] SELECTIVE DETECTION OF PHOSPHOPEPTIDES IN COMPLEX-MIXTURES BY ELECTROSPRAY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY
HUDDLESTON, MJ
ANNAN, RS
BEAN, MF
CARR, SA
[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1993, 4 (09) : 710 - 717
[10] PROTEIN SEQUENCING BY TANDEM MASS-SPECTROMETRY
HUNT, DF
YATES, JR
SHABANOWITZ, J
WINSTON, S
HAUER, CR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (17) : 6233 - 6237

← 1 2 →