FLOW-INJECTION DETERMINATION OF 1,5-ANHYDROGLUCITOL IN SERUM WITH AN IMMOBILIZED PYRANOSE OXIDASE REACTOR AND CHEMILUMINESCENCE DETECTION

被引：11

作者：

KIBA, N ^{[1
]}

UEDA, F ^{[1
]}

SAEGUSA, K ^{[1
]}

GOTO, Y ^{[1
]}

FURUSAWA, M ^{[1
]}

YAMANE, T ^{[1
]}

机构：

[1] YAMANASHI UNIV,FAC EDUC,DEPT CHEM,KOFU,YAMANASHI 400,JAPAN

来源：

ANALYTICA CHIMICA ACTA | 1993年 / 271卷 / 01期

关键词：

CHEMILUMINESCENCE; ENZYMATIC METHODS; FLOW INJECTION; ANHYDROGLUCITOL; ENZYME REACTOR; PYRANOSE OXIDASE; SERUM;

D O I：

10.1016/0003-2670(93)80550-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A flow-injection system with a separation column and an immobilized enzyme reactor is described for the determination of 1,5-anhydro-D-glucitol in serum. Serum samples diluted with water are injected into an anion-exchange column (4 cm x 4 mm i.d.). The eluate is passed through an immobilized pyranose oxidase reactor (5 cm x 4 mm i.d.). The hydrogen peroxide produced is detected chemiluminometrically with a luminol-hexacyanoferrate(III) reaction. The calibration graph is linear over the range 4 x 10(-7)-2 x 10(-4) M; the detection limit is 2 x 10(-7) M. The sample throughput is 20 h-1 with a relative standard deviation of less than 2.5%.

引用

页码：47 / 51

页数：5

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