ELECTROCHEMICAL SENSORS FOR DIRECT REAGENTLESS MEASUREMENT OF SUPEROXIDE PRODUCTION BY HUMAN NEUTROPHILS

被引：60

作者：

MCNEIL, CJ ^{[1
]}

GREENOUGH, KR ^{[1
]}

WEEKS, PA ^{[1
]}

SELF, CH ^{[1
]}

COOPER, JM ^{[1
]}

机构：

[1] UNIV GLASGOW,DEPT ELECTR & ELECT ENGN,GLASGOW G12 8QQ,SCOTLAND

来源：

FREE RADICAL RESEARCH COMMUNICATIONS | 1992年 / 17卷 / 06期

基金：

英国医学研究理事会;

关键词：

ELECTROCHEMICAL SENSORS; SUPEROXIDE; IMMOBILIZED CYTOCHROME-C; SUPEROXIDE DISMUTASE; MODIFIED ELECTRODE; ACTIVATED CARBON; NEUTROPHILS; FREE RADICALS;

D O I：

10.3109/10715769209083144

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrochemical sensors based on immobilised cytochrome c or superoxide dismutase for the measurement of superoxide radical production by stimulated neutrophils are described. Cytochrome c was immobilised covalently at a surface-modified gold electrode and by passive adsorption to novel platinised activated carbon electrodes (PACE). The reoxidation of cytochrome c at the electrode surface upon reduction by superoxide was monitored using both xanthine/xanthine oxidase and stimulated neutrophils as sources of the free radical. In addition, bovine Cu/Zn superoxide dismutase was immobilised to PACE by passive adsorption and superoxide, generated by xanthine/xanthine oxidase, detected by oxidation of hydrogen peroxide produced by the enzymic dismutation of the superoxide radical. A biopsy needle probe electrode based on cytochrome c immobilised at PACE and suitable for continuous monitoring of free radical production was constructed and characterised.

引用

页码：399 / 406

页数：8

共 9 条

[1] ENZYME OF GRANULOCYTES THAT PRODUCES SUPEROXIDE AND PEROXIDE - ELUSIVE PIMPERNEL [J].

BADWEY, JA ;

CURNUTTE, JT ;

KARNOVSKY, ML .

NEW ENGLAND JOURNAL OF MEDICINE, 1979, 300 (20) :1157-1160

[2]

BENNETTO HP, 1987, Patent No. 8707295

[3]

Churchouse S.J., 1986, ANAL P, V23, P146

[4] AMPEROMETRIC ENZYME ELECTRODE FOR THE DETERMINATION OF ASPARTATE-AMINOTRANSFERASE AND ALANINE AMINOTRANSFERASE IN SERUM [J].

COOPER, JM ;

MCNEIL, CJ ;

SPOORS, JA .

ANALYTICA CHIMICA ACTA, 1991, 245 (01) :57-62

[5]

COOPER JM, 1992, J ELECTROANALYTICAL

[6]

Fridovich, 1977, SUPEROXIDE SUPEROXID, P11

[7] THE PATHO-PHYSIOLOGY OF SUPEROXIDE - ROLES IN INFLAMMATION AND ISCHEMIA [J].

MCCORD, JM ;

ROY, RS .

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 1982, 60 (11) :1346-1352

[8] AMPEROMETRIC BIOSENSOR FOR RAPID MEASUREMENT OF 3-HYDROXYBUTYRATE IN UNDILUTED WHOLE-BLOOD AND PLASMA [J].

MCNEIL, CJ ;

SPOORS, JA ;

COOPER, JM ;

ALBERTI, KGMM ;

MULLEN, WH .

ANALYTICA CHIMICA ACTA, 1990, 237 (01) :99-105

[9] APPLICATION OF THE ELECTROCHEMISTRY OF CYTOCHROME-C TO THE MEASUREMENT OF SUPEROXIDE RADICAL PRODUCTION [J].

MCNEIL, CJ ;

SMITH, KA ;

BELLAVITE, P ;

BANNISTER, JV .

FREE RADICAL RESEARCH COMMUNICATIONS, 1989, 7 (02) :89-96

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