EXPRESSION OF SILVER-STAINED NUCLEOLAR ORGANIZER REGIONS IS COUPLED TO CELL-CYCLE IN RAT THYMIC CELLS

被引:33
作者
CARBAJO, S
ORFAO, A
VICENTEVILLARDON, JL
CARBAJOPEREZ, E
机构
[1] UNIV SALAMANCA,DEPT HUMAN ANAT & HISTOL,CYTOMETRY UNIT,E-37007 SALAMANCA,SPAIN
[2] UNIV SALAMANCA,BYOSTAT UNIT,E-37007 SALAMANCA,SPAIN
来源
CYTOMETRY | 1993年 / 14卷 / 01期
关键词
AGNORS; IMAGE PROCESSING; CELL CYCLE; FLOW CYTOMETRY; BROMODEOXYURIDINE; THYMUS; RAT;
D O I
10.1002/cyto.990140109
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
To directly analyze the relationship between the expression of silver-stained nucleolar organizer regions (AgNORs) and cell proliferation, thymic cells from newborn rats were separately sorted at the GO-G1-phase and early-mid and late-mid S-phase of the cell cycle according to their DNA content. Different AgNOR-derived parameters (mean area and numbers of AgNORs per cell and mean AgNOR-particle area) were evaluated after silver staining of cytospins. A linear correlation was observed between the mean area and numbers of AgNORs per cell, both parameters increasing progressively from GO-G1-phase to early-mid and late-mid S-phase. An increase of the mean AgNOR-particle area was also seen between GO-G1 and S-phase but this was not significant along the S-phase. A bias on the selection of S-phase cells linked to intrathymic maturation can be ruled out as S-phase cells labeled with bromodeoxyuridine were found throughout the thymus, and, moreover, the analysis of the frequency distribution of nuclear area did not show a bimodal pattern. It is concluded that the expression of AgNORs-evaluated as AgNOR area/cell or AgNOR numbers/cells-is causally or indirectly coupled to DNA synthesis and, thus, AgNORs can be considered as a cell proliferation marker.
引用
收藏
页码:46 / 52
页数:7
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