HUMAN PLATELET GLYCOPROTEIN-IIIB BINDS TO THROMBOSPONDIN FRAGMENTS BEARING THE C-TERMINAL REGION, AND OR THE TYPE-I REPEATS (CSVTCG MOTIF), BUT NOT TO THE N-TERMINAL HEPARIN-BINDING REGION

被引:15
作者
CATIMEL, B
LEUNG, L
ELGHISSASI, H
MERCIER, N
MCGREGOR, J
机构
[1] INST PASTEUR LYON, FAC MED ALEXIS CARREL, INSERM U331, F-69372 LYON 08, FRANCE
[2] STANFORD UNIV, SCH MED, DIV HEMATOL, STANFORD, CA 94305 USA
关键词
D O I
10.1042/bj2840231
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Major blood membrane platelet glycoprotein IIIb (GPIIIb), also termed GPIV or CD365, has been identified as a receptor for thrombospondin (TSP), collagen and Plasmodium falciparum-infected erythrocytes. The aim of the present study was to identify region(s) of TSP involved in binding of GPIIIb. Proteolytic fragments of TSP (M(r) 140 kDa, 120-18 kDa and 27 kDa on SDS/PAGE under reducing conditions) were purified by f.p.l.c. and identified by N-terminal gas-phase sequencing, e.l.i.s.a. and Western blots using monoclonal antibodies directed against defined domains of TSP. The 140 kDa and 120-18 kDa fragments (C-terminal region), but not the 27 kDa fragment (N-terminal region), were shown to bind to GPIIIb by using e.l.i.s.a. and affinity-chromatography systems. TSP binding to a GPIIIb-affinity column was Ca2+-dependent and reduced by 45% in the presence of EDTA. Moreover, TSP was only partially eluted with EDTA from a Ca2+-equilibrated GPIIIb column. A fragment of 68 kDa, obtained by further digestion of the 140 kDa fragment, bound to the GPIIIb-Sepharose affinity column. This fragment, or stalk-like region, bears the TSP type I repeats that show sequence similarity to regions on properdin, Plasmodium falciparum proteins and antistasin. Peptides (CSVTCG or SVTCGGGV) representing these repeats bound isolated GPIIIb in a Ca2+-independent way, but did not completely inhibit the GPIIIb and TSP interaction. These studies indicate that GPIIIb binds to the TSP via the C-terminal region and/or the CSVTCG motif, but not to the N-terminal region. Interaction between GPIIIb and the TSP C-terminal region or the CSVTCG motif is respectively Ca2+-dependent and -independent.
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页码:231 / 236
页数:6
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