MICROBIAL TRANSFORMATION OF STEROLS TO C19-STEROIDS BY RHODOCOCCUS-EQUI

被引：22

作者：

AHMAD, S ^{[1
]}

ROY, PK ^{[1
]}

KHAN, AW ^{[1
]}

BASU, SK ^{[1
]}

JOHRI, BN ^{[1
]}

机构：

[1] GOVIND BALLABH PANT UNIV AGR & TECHNOL,COLL BASIC SCI & HUMANITIES,DEPT MICROBIOL,PANTNAGAR 263145,UTTAR PRADESH,INDIA

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1991年 / 7卷 / 05期

关键词：

D O I：

10.1007/BF00368360

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A wild-type strain of Rhodococcus equi, isolated from soil, degraded cholesterol, beta-sitosterol, stigmasterol and mixed sterols to androst-4-ene-3,17-dione (AD) and androsta-1,4-diene-3,17-dione (ADD). A definite preference for a relatively simply structured cholesterol side chain was observed. Highest specific cholesterol side-chain cleavage was associated with active growth of the culture. Maximum yield of ADD was obtained when sodium acetate and cholesterol were incorporated together in the medium. Specific side-chain cleavage required the presence of 2,2'-dipyridyl, an inhibitor of ring cleavage.

引用

页码：557 / 561

页数：5

共 18 条

[1]

Atrat P., Huller E., Horhold C., Steroid transformation with immobilized microorganisms V. Continuous side chain cleavage of a cholesterol derivative with immobilized Mycobacterium phlei cells, European Journal of Applied Microbiology and Biotechnology, 12, pp. 157-160, (1981)

[2]

Choi S.K., Kim H.S., Park Y.H., Microbial conversion of cholesterol and androstadienedione by intermittent addition of substrate, Korean Journal of Applied Microbiology and Bioengineering, 16, pp. 187-192, (1988)

[3]

Coto O., Martinez J., Roboredo M.R., Carballo M.E., Quintana M., Rodriguez B., Pupo M., Microbiological transformation of steroids: II Transformation of cholesterol by bacteria, Revista de Ciencias Biologicas, 16, pp. 217-222, (1985)

[4]

Goodfellow M., Alderson G., The actinomycete-genus Rhodococcus: a home for the ‘rhodochrous’ complex, Journal of General Microbiology, 100, pp. 99-122, (1977)

[5]

Goswami P.C., Singh H.D., Hazarika A.K., Baruah J.N., Isolation and characterization of sterol metabolites formed by an Arthrobacter sp, Indian Journal of Biochemistry and Biophysics, 19, pp. 367-369, (1982)

[6]

Jankov R.M., Stefanovic M., Microbial transformation of steroids. X transformation of 19-substituted cholesterol derivative into esterone by the strain of Mycobacterium phlei, Journal of Serbian Chemical Society, 51, pp. 523-527, (1986)

[7]

Kieslich K., Production of drugs by microbial biosynthesis and biotransformation: possibilities, limits and future developments (IInd communication), Drug Research, 36, pp. 888-892, (1986)

[8]

Martin C.K.A., Wagner F., Microbial transformation of β-sitosterol by Nocardia sp. M 29, European Journal of Applied Microbiology, 2, pp. 243-255, (1976)

[9]

Martin C.K.A., Sterols, Biotechnology, a comprehensive treatise in 8 volumes, pp. 79-96, (1984)

[10]

Martinez J., Coto O., Reboredo M., Carballo M., Alfonso M., Microbial transformation of sterols from sugarcane to pharmaceutical substances, Revista Latinoamericana de Microbiologia, 29, pp. 373-376, (1987)

← 1 2 →