MICROBIAL TRANSFORMATION OF STEROLS TO C19-STEROIDS BY RHODOCOCCUS-EQUI

被引：22

作者：

AHMAD, S ^{[1
]}

ROY, PK ^{[1
]}

KHAN, AW ^{[1
]}

BASU, SK ^{[1
]}

JOHRI, BN ^{[1
]}

机构：

[1] GOVIND BALLABH PANT UNIV AGR & TECHNOL,COLL BASIC SCI & HUMANITIES,DEPT MICROBIOL,PANTNAGAR 263145,UTTAR PRADESH,INDIA

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1991年 / 7卷 / 05期

关键词：

D O I：

10.1007/BF00368360

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A wild-type strain of Rhodococcus equi, isolated from soil, degraded cholesterol, beta-sitosterol, stigmasterol and mixed sterols to androst-4-ene-3,17-dione (AD) and androsta-1,4-diene-3,17-dione (ADD). A definite preference for a relatively simply structured cholesterol side chain was observed. Highest specific cholesterol side-chain cleavage was associated with active growth of the culture. Maximum yield of ADD was obtained when sodium acetate and cholesterol were incorporated together in the medium. Specific side-chain cleavage required the presence of 2,2'-dipyridyl, an inhibitor of ring cleavage.

引用

页码：557 / 561

页数：5

共 18 条

[11]

Nagasawa M., Hashiba H., Watanabe N., Bae M., Tamura G., Arima K., Microbial transformation of sterols. Part IV, C<sub>19</sub>-steroid intermediates in the degradation of cholesterol by Arthrobacter simplex, Agricultural and Biological Chemistry, 34, pp. 801-804, (1970)

[12]

Owen R.W., Mason A.N., Bilton R.F., The degradation of cholesterol by Pseudomonas sp. NCIB 10590 under aerobic conditions, Journal of Lipid Research, 24, pp. 1500-1511, (1983)

[13]

Patil S., Mishra K., Side chain cleavage of some steroid drug precursors by Mycobacterium mutants, Indian Journal of Experimental Biology, 27, pp. 374-375, (1989)

[14]

Quintana M., Coto O., Reboredo M.R., Carballo M.E., Martinez J., Microbiological transformation of steroids: I General aspects, Revista de Ciencias Biologicas, 116, pp. 209-216, (1985)

[15]

Seki T., Separation of steroids by means of ion exchange resins, Methods in Enzymology, 15, pp. 219-237, (1969)

[16]

Smith I., Chromatographic and Electrophoretic Techniques, (1960)

[17]

Steinert H.J., Vorlop D., Klein J., Steroid side chain cleavage with immobilized living cells in organic solvents, Biocatalysis in organic media, pp. 51-63, (1987)

[18]

Zajaczkowska E., Sedlaczek L., Microbiological degradation of sterols I. Selective induction of enzymes of the cholesterol side chain cleavage in Rhodococcus sp, IM 58 Acta Microbiologica Polonica, 37, pp. 27-30, (1988)

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