A 24-AMINO-ACID POLYPEPTIDE IS ESSENTIAL FOR THE BIOSYNTHESIS OF THE COENZYME PYRROLO-QUINOLINE-QUINONE

被引：56

作者：

GOOSEN, N

HUINEN, RGM

VANDEPUTTE, P

机构：

[1] Molecular Genetics Department, Gorlaeus Laboratories, University of Leiden, 2300 RA Leiden

来源：

JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 04期

关键词：

D O I：

10.1128/jb.174.4.1426-1427.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

At least four genes are required for the biosynthesis of the coenzyme pyrrolo-quinoline-quinone (PQQ) in Acinetobacter calcoaceticus. The DNA region where one of these genes was mapped codes for a polypeptide of only 24 amino acids. Here we show that indeed this small peptide is essential for PQQ synthesis. Site-directed mutagenesis shows that at least one glutamate and one tyrosine residue of the polypeptide are essential for its function.

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页码：1426 / 1427

页数：2

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