SODIUM UPTAKE ACROSS BASOLATERAL MEMBRANE OF RAT DISTAL COLON - EVIDENCE FOR NA-H EXCHANGE AND NA-ANION COTRANSPORT

被引:66
作者
RAJENDRAN, VM [1 ]
OESTERLIN, M [1 ]
BINDER, HJ [1 ]
机构
[1] YALE UNIV,SCH MED,DEPT MED,333 CEDAR ST,NEW HAVEN,CT 06510
关键词
DIDS-SENSITIVE NA UPTAKE; DIDS-INSENSITIVE NA UPTAKE; BICARBONATE-STIMULATED NA COTRANSPORT; DIDS-SENSITIVE NA-OH COTRANSPORT;
D O I
10.1172/JCI115444
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
This study sought to characterize the mechanism of Na transport across basolateral membrane vesicles of rat distal colon. Both an outward proton gradient and an inward bicarbonate gradient stimulated Na-22 uptake. Proton gradient-stimulated Na-22 uptake was activated severalfold by the additional presence of an inward bicarbonate gradient, and bicarbonate gradient-stimulated Na-22 uptake was significantly enhanced by an imposed intravesicular membrane positive potential. 0.1 mM amiloride inhibited both proton gradient-and bicarbonate gradient-stimulated Na-22 uptake by 80 and 95%, respectively, while 1 mM 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) inhibited both proton gradient-and bicarbonate gradient-stimulated Na-22 uptake by 40 and 80%, respectively. Both proton gradient- and bicarbonate gradient-stimulated Na-22 uptake saturated as a function of increasing Na concentration: the apparent kinetic constants (K(m)) for Na for the DIDS-insensitive component of proton gradient-stimulated Na-22 uptake was 46.4 mM, while the DIDS-sensitive component of proton gradient- and bicarbonate gradient-stimulated Na-22 uptake had K(m) for Na of 8.1 and 6.4 mM, respectively. Amiloride inhibited both DIDS-insensitive proton gradient- and bicarbonate gradient-stimulated Na-22 uptake with an inhibitory constant (K(i)) of approximately 35 and 1-mu-M, respectively. We conclude from these results that proton graident-stimulated Na-22 uptake represents both DIDS-insensitive Na-H exchange and DIDS-sensitive electrogenic Na-OH cotransport, and that the DIDS-sensitive component of proton gradient-stimulated Na-22 uptake and bicarbonate gradient-stimulated Na-22 uptake may represent the same electrogenic Na-anion cotransport process.
引用
收藏
页码:1379 / 1385
页数:7
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