ULTRAFILTRATE OF SALIVA COLLECTED INSITU FOR THE MEASUREMENT OF TESTOSTERONE

被引：10

作者：

SCHRAMM, W ^{[1
]}

PAEK, SH ^{[1
]}

KUO, HH ^{[1
]}

YANG, T ^{[1
]}

机构：

[1] UNIV MICHIGAN, BIOENGN PROGRAM, ANN ARBOR, MI 48109 USA

来源：

ANALYTICA CHIMICA ACTA | 1991年 / 248卷 / 02期

关键词：

IMMUNOASSAY; OSMOTIC PUMP; SALIVA; TESTOSTERONE;

D O I：

10.1016/S0003-2670(00)84670-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A device for the in situ collection of an ultrafiltrate of saliva was investigated. The collector consists of an osmotic pump that, when placed in the mouth, accumulates a prepurified salivary filtrate within a few minutes. The concentration of testosterone in saliva and in the ultrafiltrate from five male subjects was determined by a solid-phase immunoassay. The ultrafiltrate can be used without extraction as a medium for the diagnostic evaluation of free, protein-unbound testosterone. Concentrations in whole saliva and the ultrafiltrate correlate closely (r = 0.89; n = 42). The collector can potentially be used for the measurement of a wide variety of analytes other than testosterone. An ultrafiltrate of saliva as diagnostic medium provides the following advantages: simplicity of collection; moderate stimulation of salivary flow; exclusion of potential blood contamination; prevention of binding of analytes to proteins; prevention of potential metabolic degradation of analytes; reduction of viscosity by exclusion of mucopolysaccharides and other large molecules; and potential sterile sampling of ultrafiltrate.

引用

页码：517 / 528

页数：12