HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF AMOXICILLIN IN URINE USING SOLID-PHASE, ION-PAIR EXTRACTION AND ULTRAVIOLET DETECTION

被引：21

作者：

CHULAVATNATOL, S ^{[1
]}

CHARLES, BG ^{[1
]}

机构：

[1] UNIV QUEENSLAND,DEPT PHARM,BRISBANE,QLD 4072,AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 615卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)80294-E

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple, robust high-performance liquid chromatographic method is described for assaying amoxicillin in urine. Sample clean-up involved solid-phase, ion-pair extraction onto Sep-Pak C18 cartridges followed by elution with acetonitrile (3%, v/v) in pH 4.85 buffer. Separations were performed on an Ultrasphere C18 column with a mobile phase comprising acetonitrile (32.5 ml) and pH 7.1 phosphate buffer (0.01 M, 1000 ml). Peaks were detected at 229 nm. Recovery was greater than 94%. Plots of peak area against urinary amoxicillin concentration were linear (r > 0.999) from 5 to 500 mg/l. Between-day and within-day imprecision (coefficient of variation) ranged between 3.0 and 15.5%. Inaccuracy was 7.1%, or less.

引用

页码：91 / 96

页数：6