SPECIFIC ASSOCIATION BETWEEN THE HUMAN DNA-REPAIR PROTEINS XPA AND ERCC1

被引：274

作者：

LI, L

ELLEDGE, SJ

PETERSON, CA

BALES, ES

LEGERSKI, RJ

机构：

[1] UNIV TEXAS,MD ANDERSON CANC CTR,DEPT MOLEC GENET,HOUSTON,TX 77030

[2] BAYLOR COLL MED,HOWARD HUGHES MED INST,DEPT BIOCHEM,HOUSTON,TX 77030

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 11期

关键词：

XERODERMA PIGMENTOSUM; NUCLEOTIDE EXCISION REPAIR; PROTEIN-PROTEIN INTERACTION;

D O I：

10.1073/pnas.91.11.5012

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Processing of DNA damage by the nucleotide-excision repair pathway in eukaryotic cells is most likely accomplished by multiprotein complexes. However, the nature of these complexes and the details of the molecular interactions between DNA repair factors are for the most part unknown. Here, we demonstrate both in vivo, using the two-hybrid system, and in vitro, using recombinant proteins, that the human repair factors XPA and ERCC1 specifically interact. In addition, we report an initial determination of the domains in ERCC1 and XPA that mediate this interaction. These results suggest that XPA may play a role in the localization or loading of an incision complex, composed of ERCC1 and possibly other repair factors, onto a damaged site.

引用

页码：5012 / 5016

页数：5

共 34 条

[31] ERCC2 - CDNA CLONING AND MOLECULAR CHARACTERIZATION OF A HUMAN NUCLEOTIDE EXCISION REPAIR GENE WITH HIGH HOMOLOGY TO YEAST RAD3 [J].