SEQUENCE-INDEPENDENT DETECTION OF GENE FAMILY HOMOLOGS - IDENTIFICATION OF A TRANSCRIPT ENCODING A MOLLUSCAN SERINE-PROTEASE HOMOLOGOUS TO THE PANCREATIC-ENZYMES OF VERTEBRATES

被引：2

作者：

GROPPE, JC

MORSE, DE

机构：

[1] UNIV CALIF SANTA BARBARA,DEPT BIOL SCI,SANTA BARBARA,CA 93106

[2] UNIV CALIF SANTA BARBARA,CTR MARINE BIOTECHNOL,SANTA BARBARA,CA 93106

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1995年 / 110卷 / 01期

关键词：

CDNAS; GENE FAMILY HOMOLOGS; TRANSCRIPT; MOLLUSCAN SERINE PROTEASE; PANCREATIC ENZYMES;

D O I：

10.1016/0305-0491(94)00142-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Autoradiography of P-32-labeled cDNA, fractionated at high resolution by electrophoresis through thin (0.8-1.5 mm) vertical alkaline agarose gels, provides a sequence-independent screening procedure for gene family homologs, A screen of tissues of a marine mollusc revealed a prominent intestine-specific cDNA encoding a pancreatic serine protease homolog, which was not detectable as a discrete poly(A)(+) RNA species on formaldehyde agarose gels, Discrete cDNA products are authentic, non-truncated transcripts of tissue-specific mRNA. A band-sharpening effect is imparted to cDNA products due to (a) substitution of a uniform length 5'-oligo(dT) terminus for heterogeneous 3'-poly(A) termini rind (b) the inherent superior resolution of alkaline-denatured DNA,

引用

页码：75 / 82

页数：8

共 22 条

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GROPPE JC, 1989, 1ST P INT MAR BIOT C, P285

[12]

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