INTERPHASE CYTOGENETICS USING BIOTIN AND DIGOXIGENIN LABELED PROBES .3. INCREASED SENSITIVITY AND FLEXIBILITY FOR DETECTING HPV IN CERVICAL BIOPSY SPECIMENS AND CELL-LINES

被引：65

作者：

HERRINGTON, CS ^{[1
]}

GRAHAM, AK ^{[1
]}

MCGEE, JO ^{[1
]}

机构：

[1] UNIV OXFORD, JOHN RADCLIFFE HOSP, NUFFIELD DEPT PATHOL & BACTERIOL, OXFORD OX3 9DU, ENGLAND

来源：

JOURNAL OF CLINICAL PATHOLOGY | 1991年 / 44卷 / 01期

关键词：

D O I：

10.1136/jcp.44.1.33

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

A monoclonal antibody to digoxin enabled sandwich techniques to be used for the detection of hybridised digoxigenin labelled probes in cultured cells and paraffin wax sections. This system has greater flexibility than alkaline phosphatase conjugated polyclonal antidigoxigenin antibody and permits the use of alternative detector enzymes, such as horseradish peroxidase and fluorescence labels. The APAAP detection system that does not require the use of biotin can also be used in situations where endogenous biotin is a problem. The low level of background staining combined with precise substrate deposition of the amplified peroxidase system gives higher sensitivity and resolution. This permits localisation of closely adjacent chromosomal loci in interphase nuclei. The most sensitive peroxidase based digoxigenin detection system visualises two and a half to 12 copies of human papillomavirus (HPV) per nucleus. This system is also suitable for the analysis of low copy number HPV infection of cervical tissues.

引用

页码：33 / 38

页数：6

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