EXON-2 DNA-SEQUENCE OF THE HLA-DRW13B ALLELE OBTAINED FROM GENOMES OF 5 DIFFERENT INDIVIDUALS

被引：32

作者：

CORELL, A ^{[1
]}

MARTINVILLA, JM ^{[1
]}

VARELA, P ^{[1
]}

VICARIO, JL ^{[1
]}

MARTINEZLASO, J ^{[1
]}

ARNAIZVILLENA, A ^{[1
]}

机构：

[1] COMUNIDAD AUTONOMA MADRID,CTR TRANSFUS,MADRID,SPAIN

来源：

MOLECULAR IMMUNOLOGY | 1990年 / 27卷 / 03期

关键词：

D O I：

10.1016/0161-5890(90)90145-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Exon 2 nucleotide sequence of the DRB1 gene encoding the HLA-DRw13b allele defined by DNA-RFLP (Restriction Fragment Length Polymorphism) typing, has been obtained by using five heterozygous individuals genomic DNA and a non isotopic automated "dideoxi" methodology. Its comparison with other known homologous DRB1 sequences suggests that two different mechanisms which generate HLA allele variability may have occurred in this particular exon 2: a gene conversion between DRw11 or DRw13 as acceptors and DR4-Dw15 or DRw8.1 as donors and in addition, a non-conservative point mutation at base 221. The relationship between this HLA sequence characteristics and certain diseases susceptibility is discussed. © 1990.

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页码：313 / 316

页数：4

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