ANALYSIS OF NUCLEAR PROTEINS INTERACTING WITH A WHEAT ALPHA/BETA-GLIADIN SEED STORAGE PROTEIN GENE

被引:7
作者
VELLANOWETH, RL [1 ]
OKITA, TW [1 ]
机构
[1] WASHINGTON STATE UNIV,INST BIOL CHEM,PULLMAN,WA 99164
关键词
GLIADIN STORAGE PROTEINS; WHEAT; TRANSCRIPTIONAL REGULATION; NUCLEAR PROTEINS;
D O I
10.1007/BF00038993
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The promoter region ( -524 to -46) of the wheat alpha/beta-gliadin seed storage protein gene was analyzed for interactions with nuclear proteins from developing wheat seeds. Six complexes were detected within the first 165 bp upstream of the transcriptional start site. One of the proteins was a non-sequence specific AT-binding protein. The remaining five proteins bound in a sequence specific manner. One (CABP) mapped to a conserved CA-rich element at -134 to -112 while another (PalBP) mapped to an adjacent, palindromic sequence at -112 to -106. Three proteins (CTBPs 1-3) formed complexes at two, independent homologous sites. The activities of four of the binding proteins, CTBPs 1-3 and CABP, exhibited similar patterns of expression during seed development: they first appeared at early to mid stages, reached a maximum at mid stage and subsequently decreased, paralleling the pattern of gliadin mRNA accumulation. The non-specific AT-binding protein was detected at relatively high levels only at mid development. PalBP activity, on the other hand, first appeared at mid stage and was present at a constant level throughout later stages of development. The results suggest that the binding proteins may regulate gliadin expression in an antagonistic manner.
引用
收藏
页码:25 / 41
页数:17
相关论文
共 39 条
[1]   NUCLEAR FACTORS INTERACT WITH A SOYBEAN BETA-CONGLYCININ ENHANCER [J].
ALLEN, RD ;
BERNIER, F ;
LESSARD, PA ;
BEACHY, RN .
PLANT CELL, 1989, 1 (06) :623-631
[2]   NUCLEIC-ACID SEQUENCE AND CHROMOSOME ASSIGNMENT OF A WHEAT STORAGE PROTEIN GENE [J].
ANDERSON, OD ;
LITTS, JC ;
GAUTIER, MF ;
GREENE, FC .
NUCLEIC ACIDS RESEARCH, 1984, 12 (21) :8129-8144
[3]   STRUCTURAL AND FUNCTIONAL-ANALYSIS OF PROMOTER FROM GLIADIN, AN ENDOSPERM-SPECIFIC STORAGE PROTEIN GENE OF TRITICUM-AESTIVUM L [J].
ARYAN, AP ;
AN, GH ;
OKITA, TW .
MOLECULAR AND GENERAL GENETICS, 1991, 225 (01) :65-71
[4]   THE CHARACTERIZATION OF CDNA CLONES CODING FOR WHEAT STORAGE PROTEINS [J].
BARTELS, D ;
THOMPSON, RD .
NUCLEIC ACIDS RESEARCH, 1983, 11 (10) :2961-2977
[5]   THE CAULIFLOWER MOSAIC VIRUS-35S PROMOTER - COMBINATORIAL REGULATION OF TRANSCRIPTION IN PLANTS [J].
BENFEY, PN ;
CHUA, NH .
SCIENCE, 1990, 250 (4983) :959-966
[6]   PRIMARY STRUCTURE OF A B1 HORDEIN GENE FROM BARLEY [J].
BRANDT, A ;
MONTEMBAULT, A ;
CAMERONMILLS, V ;
RASMUSSEN, SK .
CARLSBERG RESEARCH COMMUNICATIONS, 1985, 50 (06) :333-345
[7]   FRACTIONATION OF WHEAT GLIADIN AND GLUTENIN SUBUNITS BY TWO-DIMENSIONAL ELECTROPHORESIS AND THE ROLE OF GROUP-6 AND GROUP-2 CHROMOSOMES IN GLIADIN SYNTHESIS [J].
BROWN, JWS ;
FLAVELL, RB .
THEORETICAL AND APPLIED GENETICS, 1981, 59 (06) :349-359
[8]  
BROWN JWS, 1986, EUR J CELL BIOL, V42, P161
[9]   POSITIVE AND NEGATIVE CIS-ACTING DNA DOMAINS ARE REQUIRED FOR SPATIAL AND TEMPORAL REGULATION OF GENE-EXPRESSION BY A SEED STORAGE PROTEIN PROMOTER [J].
BUSTOS, MM ;
BEGUM, D ;
KALKAN, FA ;
BATTRAW, MJ ;
HALL, TC .
EMBO JOURNAL, 1991, 10 (06) :1469-1479
[10]   A GAMMA-HORDEIN GENE [J].
CAMERONMILLS, V ;
BRANDT, A .
PLANT MOLECULAR BIOLOGY, 1988, 11 (04) :449-461